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Annexin V-APC / PI Apoptosis Detection Kit[217]

  • Cat.No.:E-CK-A217

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Introduction

Elabscience® Annexin V-APC / PI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy.

Propidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.

Components

Cat. Products 20 Assays 50 Assays 100 Assays 200 Assays Storage
E-CK-A117 Annexin V-APC Reagent[117] 100 μL 250 μL 500 μL 1 mL 2~8°C
E-CK-A151 Annexin V Binding Buffer (10 ×) 1.4 mL×2 5.5 mL 11 mL 11 mL×2 2~8°C
E-CK-A161 Propidium Iodide(PI) Staining Solution[161] 100 μL 250 μL 500 μL 1 mL 2~8°C
Manual One Copy

Jurkat cells were treated with 5 μM Camptothecin and detected with this kit.

Annexin V-APC / PI Apoptosis Detection Kit-Elabscience

Jurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-APC single-positive cells were early apoptotic cells, Annexin V-APC and PI double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.

Instructions

Dilute Annexin V Binding Buffer (10 ×) with DI water to 1×Annexin V Binding working solution before use.
For example, take 1 mL Annexin V Binding Buffer (10 ×) [E-CK-A151] and add it to 9 mL DI water to get 10 mL Annexin V Binding working solution.

Storage

Store at 2~8°C for one year. Annexin V-APC and PI Staining Solution should be stored in the dark.  

Cautions

1.For maximal assay performance, this kit should be used within 12 months. Avoid freeze / thaw cycles.

2.For FCM analysis, please set untreated cells stained with both Annexin V-APC and PI as negative control. As for compensation controls, please use drug-treated cells stained with either Annexin V-APC or PI.

3.Annexin V-APC can be detected in APC channel while ECD channel is preferred to PE channel for PI dete.

4.Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.

5.Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.

6.For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Molecular Nutrition & Food Research (2020) IF: 5.309
    Lactobacillus Protects Against S. Typhimurium-Induced Intestinal Inflammation by Determining the Fate of Epithelial Proliferation and Differentiation

    DOI: 10.1002/mnfr.201900655

    PMID: 31953989

    Sample: Cell sample
  2. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Knockdown of enhancer of rudimentary homolog inhibits proliferation and metastasis in ovarian cancer by regulating epithelial-mesenchymal transition

    DOI: 10.1016/j.biopha.2020.109974

    PMID: 32036222

    Sample: Cell culture supernatant
  3. International Journal of Medical Sciences (2020) IF: 2.523
    Down-regulation of Xist and miR-7a-5p improves LPS-induced myocardial injury

    DOI: 10.7150/ijms.45408

    PMID: 33029099

    Sample: Cell sample
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