|Alternate Names||Forkhead box protein P3,IPEX,JM2,Foxp3|
|Background||FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.|
|Clone No.||150D (See Other Available Formats)|
|Isotype||Mouse IgG1, κ|
|Isotype Control||Mouse IgG1, κ Isotype Control(PE Conjugated)[MOPC-21] [Product E-AB-F09792D]|
|Reactivity||Human, Mouse, Rat|
|Application||ICFCM (See the Application Description)|
|Storage Buffer||PBS with 0.05% Proclin300, 1% BSA|
|Shipping||Biological ice pack at 4 ℃|
|Stability & Storage||Keep as concentrated solution.|
Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
C57BL/6 splenocytes are surface stained with Anti-Mouse CD4 Monoclonal Antibody(APC Conjugated) followed by fixation and permeabilization using the Foxp3 Staining/Transcription Factor Buffer Set and intracellular staining with Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(PE Conjugated)
|Catalog No.||Product Name||Sizes|
|E-AB-F1208C||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(FITC Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208D||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(PE Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208E||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(APC Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208G||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(PE/Cyanine5 Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208H||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(PE/Cyanine7 Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208I||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(PE/Cyanine5.5 Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208J||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208L||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(AF488 Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|
|E-AB-F1208M||Anti-Human/Mouse/Rat Foxp3 Monoclonal Antibody(AF647 Conjugated)[150D]||20 Tests, 50 Tests, 100 Tests, 200 Tests|