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Cat.No.:E-BC-K236-S
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Protocols for metabolism assays
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Synonyms | AST,GOT |
Sample type | Serum, plasma, animal tissue |
Sensitivity | 0.38 IU/L |
Detection range | 0.38-72.30 IU/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 100 min |
Precision | Average inter-assay CV: 4.900%Average intra-assay CV: 4.200% |
Other instruments required | Test tube, Micropipettor, 37℃ Water bath, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.38-72.30 IU/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human plasma |
1 |
Human serum |
1 |
10% Rat liver tissue homogenate |
16-32 |
HepG2 supernatant |
1 |
Rat serum |
1 |
Mouse plasma |
1 |
10% Rat kidney tissue homogenate |
1 |
10% Rat heart tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl).
DOI: 10.1002/hep.29573 |
|
Sample: serum |
Sample: plasma |
PMID: 33360192 |
PMID: 34448397 |
Sample: plasma |
PMID: 36103835 |
Sample: serum |
Sample: blood |
DOI: 10.1111/cpr.13332 |
|
Sample: serum |
PMID: 34757230 |
Sample: serum |
PMID: 36293338 |
Sample: blood |
PMID: 36068769 |
Sample: Serum |
Sample: serum |
PMID: 36230124 |
Sample: serum |
Sample: serum |
PMID: 34681767 |
Sample: blood |
PMID: 35443217 |
Sample: serum |
PMID: 33827384 |
Sample: serum |
Sample: serum |
Sample: serum |
DOI: 10.1155/2022/9615674 |
|
PMID: 35692375 |
Sample: plasma |
PMID: 35010758 |
Sample: plasma |
PMID: 35298730 |
Sample: serum |
PMID: 35277165 |
Sample: serum |
DOI: 10.1155/2022/7832540 |
|
Sample: hepatic tissue,serum |
DOI: 10.7717/peerj.13082 |
|
PMID: 35310165 |
Sample: plasma |
Sample: serum |
DOI: 10.5650/jos.ess22033 |
|
PMID: 35965085 |
Sample: liver |
Sample: plasma |
Sample: plasma |