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Current Position:Home>>Kits>>Metabolism Assays>> Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Citations(29)

  • Cat.No.:E-BC-K236-S

  • Detection instrument: Spectrophotometer(505 nm)

Manual MSDS

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Detection principle

AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Performance characteristics

Synonyms AST,GOT
Sample type Serum, plasma, animal tissue
Sensitivity 0.38 IU/L
Detection range 0.38-72.30 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 4.900%Average intra-assay CV: 4.200%
Other instruments required Test tube, Micropipettor, 37℃ Water bath, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.38-72.30 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma

1

Human serum

1

10% Rat liver tissue homogenate

16-32

HepG2 supernatant

1

Rat serum

1

Mouse plasma

1

10% Rat kidney tissue homogenate

1

10% Rat heart tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl).

Citations

  1. HEPATOLOGY (2018) IF: 14.079
    MicroRNA 15a/16‐1 suppresses aryl hydrocarbon receptor–dependent interleukin‐22 secretion in CD4+ T cells and contributes to immune‐mediated organ injury

    DOI: 10.1002/hep.29573
    Sample: serum
  2. ENVIRONMENTAL SCIENCE & TECHNOLOGY (2022) IF: 9.028
    Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis

    DOI: 10.1021/acs.est.2c00516
    Sample: plasma
  3. JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038
    The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis

    DOI: 10.1016/j.jhazmat.2020.124876

    PMID: 33360192
  4. ACS Applied Materials & Interfaces (2021) IF: 9.2289
    Synergistic Multimodal Cancer Therapy Using Glucose Oxidase@CuS Nanocomposites

    DOI: 10.1021/acsami.1c12235

    PMID: 34448397

    		 Sample: plasma
  5. Cell Reports (2022) IF: 9.9949
    Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice

    DOI: 10.1016/j.celrep.2022.111321

    PMID: 36103835

    		 Sample: serum
  6. ACS Applied Materials & Interfaces (2019) IF: 8.456
    Multifunctional Graphene-Oxide-Reinforced Dissolvable Polymeric Microneedles for Transdermal Drug Delivery

    DOI: 10.1021/acsami.9b19518
    Sample: blood
  7. CELL PROLIFERATION (2022) IF: 8.7550
    Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

    DOI: 10.1111/cpr.13332
    Sample: serum
  8. Acta Biomaterialia (2021) IF: 8.9469
    An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo

    DOI: 10.1016/j.actbio.2021.10.041

    PMID: 34757230

    		 Sample: serum
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models

    DOI: 10.3390/ijms232012485

    PMID: 36293338

    		 Sample: blood
  10. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Activation of α7nAChR preserves intestinal barrier integrity by enhancing the HO-1 / STAT3 signaling to inhibit NF-κB activation in mice

    DOI: 10.1016/j.biopha.2022.112733

    PMID: 36068769

    		 Sample: Serum
  11. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: serum
  12. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: serum
  13. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Glabridin inhibits liver fibrosis and hepatic stellate cells activation through suppression of inflammation and oxidative stress by activating PPARγ in carbon tetrachloride-treated mice

    DOI: 10.1016/j.intimp.2022.109433
    Sample: serum
  14. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Atorvastatin Ester Regulates Lipid Metabolism in Hyperlipidemia Rats via the PPAR-signaling Pathway and HMGCR Expression in the Liver

    DOI: 10.3390/ijms222011107

    PMID: 34681767
  15. Food Bioscience (2022) IF: 4.24
    Fatty acids of Pleurotus florida mushroom: Potential molecules for blood glucose control

    DOI: 10.1016/j.fbio.2022.101558
    Sample: blood
  16. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    		 Sample: serum
  17. AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
    Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

    DOI: 10.1142/S0192415X21500452

    PMID: 33827384

    		 Sample: serum
  18. Frontiers in Immunology (2019) IF: 4.716
    Gal-3 Deficiency Suppresses Novosphyngobium aromaticivorans Inflammasome Activation and IL-17 Driven Autoimmune Cholangitis in Mice

    DOI: 10.3389/fimmu.2019.01309
    Sample: serum
  19. Scientific Reports (2022) IF: 4.996
    Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

    DOI: 10.1038/s41598-022-21816-x
    Sample: serum
  20. Cardiovascular Therapeutics (2022) IF: 3.023
    L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway

    DOI: 10.1155/2022/9615674

    PMID: 35692375

    		 Sample: plasma
  21. International Journal of Environmental Research and Public Health (2022) IF: 3.39
    Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

    DOI: 10.3390/ijerph19010498

    PMID: 35010758

    		 Sample: plasma
  22. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: serum
  23. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues

    DOI: 10.1186/s12906-022-03536-x

    PMID: 35277165

    		 Sample: serum
  24. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540
    Sample: hepatic tissue,serum
  25. PeerJ (2022) IF: 2.984
    3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis

    DOI: 10.7717/peerj.13082

    PMID: 35310165

    		 Sample: plasma
  26. Open Life Sciences (2022) IF: 1.311
    β-Hydroxybutyrate upregulates FGF21 expression through inhibition of histone deacetylases in hepatocytes

    DOI: 10.1515/biol-2022-0095
    Sample: serum
  27. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    		 Sample: liver
  28. Toxicology Reports (2022)
    Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

    DOI: 10.1016/j.toxrep.2022.06.005
    Sample: plasma
  29. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002
    Sample: plasma
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