β-agonist(beta-agonist) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E076

      Size:
      • 96T
      Qty:
      - +
      Price: $392

      sample_type: Feed

      Sensitivity: 0.1 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect β-agonists in samples, such as feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, β-agonist in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-β-agonist antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of β-agonist. The concentration of β-agonist in the samples can be calculated by comparing the OD of the samples to the standard curve.



      Technical indicator

      Sensitivity: 0.1 ppb (ng/mL)

      Reaction mode: 25℃, 30 min~15 min

      Detection limit: Feed---10 ppb

      Cross-reactivity:

      Salbutamol

      100%

      Clenbuterol

      80%

                           Ractopamine

      40%

      Brombuterol

      100%

      Terbutaline

      50%

      Cimaterol, Nadolol

      5%

      Propranolol, Oxprenolol, Epinephrine

      <1%

      Atenolol, Normodyne, 

      <1%

      Sample recovery rate: 90%±30%.


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1.5 mL each

      (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)

      HRP Conjugate Solution

      8 mL

      11×Concentrated HRP Conjugate

      1 mL

      Feed Solution

      30 mL

      Substrate Reagent A

      7 mL

      Substrate Reagent B

      7 mL

      Stop Solution

      7 mL

      20×Concentrated Wash Buffer

      25 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy

      Other materials required but not supplied

      Instrument: Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01g).

      High-precision transferpettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 

      Reagents:  Concentrated HCl, NaOH.


      Storage and valid period

      Store at 2~8℃ for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.

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