BP (Benzylpenicillin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E098

      Size:
      • 96T
      Qty:
      - +
      Price: $370

      sample_type: Tissue,Raw milk

      Sensitivity: 0.3 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect BP (Benzylpenicillin) in samples, such as tissue, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, BP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- BP antibody Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of BP. The concentration of BP in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity:0.3 ppb (ng/mL)

      Reaction mode: 25℃, 30 min~ 30 min ~ 15 min

      Detection limit: Tissue ---3 ppb; Duck---5ppb; Raw Milk---1ppb.

      Cross-reactivity: Benzylpenicillin ---100%; Ampicillin ---55%; Amoxicillin----620%;

                                    Nafcillin---15%; Oxacillin---44%; Cloxacillin---39%; Dicloxacillin ---73%

                                    Azlocillin---91%; Methicillin---32%; Cephalexin---1%.

      Sample recovery rate:  90%±30%


      Kits components



      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb, 24.3 ppb)

      HRP Conjugate

      12 mL

      Antibody Working Solution

      7 mL

      Substrate Reagent A

      7 mL

      Substrate Reagent B

      7 mL

      Stop Solution

      7 mL

      20×Concentrated Wash Buffer

      25 mL

      5×Concentrated Sample Solution 1

      40 mL

      50×Concentrated Sample Solution 2

      10 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy





      Other materials required but not supplied

      Instruments: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).

      High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: ZnSO4·7H2O



      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.
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