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Cat.No.:E-BC-K168-M
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Protocols for metabolism assays
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Synonyms | TP |
Sample type | Serum,plasma,animal tissue and cell |
Sensitivity | 0.046 mg/mL |
Detection range | 0.046-0.6 mg/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 8.200%Average intra-assay CV: 3.200% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | Reagent 2: RT, Reagent 1: 2-8℃ |
Valid period | 12 months |
It is
recommended to take 2~3 samples with expected large difference to do
pre-experiment before formal experiment and dilute the sample according to the
result of the pre-experiment and the detection range ( MERGEFIELD Detection_range 0.046-0.6
mg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type
Dilution factor
Human serum
90-110
Human plasma
90-110
Rabbit serum
90-110
Rat plasma
90-110
Chicken serum
90-110
10% Mouse kidney tissue homogenate
15-20
10% Mouse lung tissue homogenate
15-20
10% Rat spleen tissue homogenate
15-20
10% Rat heart tissue homogenate
15-20
10% Rat liver tissue homogenate
20-25
293T
1
HL-60
1
Note: The
diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
DOI: 10.5650/jos.ess22033 |
|
PMID: 35965085 |
Sample: serum |
M*****aSubmitted [ Aug 31 2022 ]
Asked: Can the plate in this kit be replaced with our own 96-well plate?
Reply
adminSubmitted [ Aug 31 2022 ]
Answered: Yes, it can be replaced with the same type of microplate.
M*****aSubmitted [ Aug 17 2022 ]
Asked: Can the kit work on plant sample?
Reply
adminSubmitted [ Aug 17 2022 ]
Answered: No, it can not detect the plant sample, But there is E-BC-K318-M which is suitable for plant tissue.