C/EBP Antibody Sampler Kit

    • C/EBP Antibody Sampler Kit -Elabscience
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    • C/EBP Antibody Sampler Kit -Elabscience
    • C/EBP Antibody Sampler Kit -Elabscience

      Catalog number:E-AB-K1456

      Size:
      • 7*20μL
      Qty:
      - +
      Price: $386

      Applications: WB

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      Product Includes

      Product name Specifications Application
      CEBP alpha Polyclonal Antibody 20μL WB,IHC-p,ELISA
      Phospho-CEBP alpha (Thr230) Polyclonal Antibody 20μL WB,ELISA
      Phospho-CEBP alpha (Ser21) Polyclonal Antibody 20μL WB,IHC-p,ELISA
      CEBP beta Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      Phospho-CEBP beta (Thr235) Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      CEBPD Polyclonal Antibody 20μL WB,IHC,ELISA
      CHOP Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated) 120μL WB,IHC,ELISA

      Product Photos

      Western Blot analysis of 293T cells with CEBP alpha Polyclonal Antibody

      Western Blot analysis of HepG2 cells with Phospho-CEBP alpha (Thr230) Polyclonal Antibody

      Western Blot analysis of HepG2 cells with Phospho-CEBP alpha (Ser21) Polyclonal Antibody

      Western Blot analysis of various cells using CEBP beta Polyclonal Antibody at dilution of 1:1000.

      Western Blot analysis of HepG2 cells using Phospho-CEBP beta (Thr235) Polyclonal Antibody at dilution of 1:500

      Western Blot analysis of LO2 cell using CEBPD Polyclonal Antibody at dilution of 1:300

      Western Blot analysis of VEC cells using CHOP Polyclonal Antibody at dilution of 1:500.

      Product description

      The C/EBP Antibody Sampler Kit provides an economical means of evaluating the C/EBP family of transcription factors and several phosphorylation sites that are involved in its activation. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

      Background

      CCAAT/enhancer-binding proteins (C/EBPs) are transcription factors critical for cellular differentiation, terminal function, and inflammatory response. Six characterized family members (C/EBPα, β, δ, γ, ε, and ζ) are distributed in a variety of tissues. Translation from alternative start codons results in two C/EBPα isoforms (p42 and p30) that are strong transcriptional activators. Research studies indicate that insulin and insulin-like growth factor-I stimulate C/EBPα dephosphorylation, which may play a key role in insulin-induced repression of GLUT4 transcription. Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 may be required for adipogenesis. The two forms of C/EBPβ, 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP), may result from alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may inhibit C/EBPβ transcriptional activity. Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity. Phosphorylation at the rat-specific site Ser105 in C/EBPβ appears essential for C/EBPβ activation in rat. C/EBPδ protein is highly expressed in adipose tissue, lung, and intestine. Increased expression of C/EBPδ mRNA levels during adipogenesis suggests that C/EBPδ plays an important role in positively regulating adipogenesis. C/EBPδ is expressed in the mammalian nervous system and plays an important role in long-term memory. CHOP is a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner. CHOP expression is induced by cellular stresses, including starvation; induced CHOP suppresses cell cycle progression from G1 to S phase. During ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death.

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