Origin and General Characteristics
||Caco-2 Cell Line
||Homo sapiens, Human
||Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ.
Culture Conditions and Handling
|Complete Growth Medium
||MEM +20% FBS +1% P/S
||Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
||Every 2 to 3 days
||Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
||Atmosphere: Air, 95%; CO2, 5%
Special Features of the Cell Line
||Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ).
||This cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF).
||Keratin, Retinoic Acid Binding Protein 1, Retinol Binding Protein 2
Recommendations for handling of cryopreserved cells