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Catalog number:EP-CL-0050
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Add to cart Compare Bulk requestCell Name | Caco-2 Cell Line |
Organism | Homo sapiens, Human |
Age | 72 years |
Tissue | Colon |
Morphology | Epithelial |
Growth Properties | Adherent |
Descriptions | Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ. |
Biosafety Level | 1 |
Complete Growth Medium | MEM +20% FBS +1% P/S |
Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
Subcultivation Ratio | 1:2-1:4 |
Medium Renewal | Every 2 to 3 days |
Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
Tumorigenic | Yes |
Effects | Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ). |
Receptor Expression | This cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF). |
Gene Expression | Keratin, Retinoic Acid Binding Protein 1, Retinol Binding Protein 2 |
Applications | Transfection host. |
Recommendations for handling of cryopreserved cells