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Caco-2 Cell Line

  • Cat.No.:EP-CL-0050

To Purchase EP-CL-0050

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  • 1×10⁶ cells/vial
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Origin and General Characteristics

Cell Name Caco-2 Cell Line
Organism Homo sapiens, Human
Age 72 years, male
Tissue Colon
Morphology Epithelial
Growth Properties Adherent
Descriptions Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ.
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium MEM +20% FBS +1% P/S
(Caco-2 Cell Line complete medium (EP-ML-0050) is recommended.)
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1:2-1:4
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Special Features of the Cell Line

Tumorigenic Yes
Effects Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ).
Receptor Expression This cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF).
Gene Expression Keratin, Retinoic Acid Binding Protein 1, Retinol Binding Protein 2

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