|Catenin alpha 1/2 Polyclonal Antibody||20μL||WB,IHC-p,IF,ELISA||H,M,R||Rabbit|
|N-Cadherin Polyclonal Antibody||20μL||WB,IHC-p,IF,ELISA||H,M,R||Rabbit|
|E-Cadherin Polyclonal Antibody||20μL||WB,IHC-p,IF,ELISA||H||Rabbit|
|P-Cadherin Polyclonal Antibody||20μL||WB,IHC-p,ELISA||H||Rabbit|
|Catenin beta Polyclonal Antibody||20μL||WB,IHC-p,IF,ELISA||H,M,R||Rabbit|
|Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated)||120μL||WB,IHC,ELISA||Goat|
Western Blot analysis of Hela cells using Catenin alpha 1/2 Polyclonal Antibody at dilution of 1:500.
Western Blot analysis of Jurkat cells using N-cadherin Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of various cells using E-cadherin Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of K562 cells with P-cadherin Polyclonal Antibody.
Western Blot analysis of 823 cells using Catenin beta Polyclonal Antibody at dilution of 1:2000.
This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis.