Calu-3 Cell Line


      Catalog number:EP-CL-0054

      • 1×10⁶ cells/vial
      - +
      Price: $401

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      Origin and General Characteristics

      Cell Name Calu-3 Cell Line
      Organism Homo sapiens, Human
      Age 25 years
      Tissue Lung adenocarcinoma, derived from metastatic site: pleural effusion
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions The patient had received prior therapy with cytoxan, bleomycin and adriamycin.
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium MEM +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Special Features of the Cell Line

      Tumorigenic Yes
      Effects Yes, forms well differentiated grade I adenocarcinoma in nude mice.
      Antigen Expression Antigen expression: Blood Type A; Rh+
      Gene Expression Blood Type A; Rh+
      Applications Transfection host.

      Related Resources

      Recommendations for handling of cryopreserved cells

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