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Calu-3 Cell Line

  • Cat.No.:EP-CL-0054

To Purchase EP-CL-0054

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  • 1×10⁶ cells/vial
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Origin and General Characteristics

Cell Name Calu-3 Cell Line
Organism Homo sapiens, Human
Age 25 years
Tissue Lung adenocarcinoma, derived from metastatic site: pleural effusion
Morphology Epithelial
Growth Properties Adherent
Descriptions The patient had received prior therapy with cytoxan, bleomycin and adriamycin.
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium MEM +10% FBS +1% P/S
(Calu-3 Cell Line complete medium (EP-ML-0054) is recommended.)
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1:2-1:4
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Special Features of the Cell Line

Tumorigenic Yes
Effects Yes, forms well differentiated grade I adenocarcinoma in nude mice.
Antigen Expression Antigen expression: Blood Type A; Rh+
Gene Expression Blood Type A; Rh+

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