CAPN6 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human; Mouse; Rat
- Applications: WB
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Verified Samples |
Verified Samples in WB:Rat heart,A172,NIH/3T3,TM4 |
Dilution |
WB 1:500-1:2000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Fusion protein of human CAPN6 |
Abbre | CAPN6 |
Synonyms | CALP M;Calpain-6;Calpain-like protease X-linked;Calpamodulin;CalpM;CAN6;CANP X;CANPX;CAPN 6;Capn6 |
Swissprot | |
Calculated MW | 75 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm>perinuclear region. Cytoplasm>cytoskeleton>spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle. |
Concentration | 0.78 mg/mL |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Cancer; Cell Biology; Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. |