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CASP9 Polyclonal Antibody

Cat:E-AB-30760
Manual MSDS

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Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
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Product Details
Verified Samples Verified Samples in WB:L929,Rat muscle,Mouse brain,Rat kidney
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from human Caspase-9 around the non-phosphorylation site of Thr125.
Abbre Caspase-9
Synonyms APAF-3;APAF3;Apoptosis related cysteine peptidase;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;CASP-9;CASP9;CASP9;Caspase 9 apoptosis related cysteine peptidase;Caspase 9 Dominant Negative;Caspase 9c;Caspase-9;Caspase-9 subunit p10;ICE LAP6;ICE like apoptotic protease 6;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1;regulatory subunit 56;RNCASP9
Swissprot
Calculated MW 46kDa
Observed MW 46kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytosol,apoptosome,cytosol,Mitochondrion,Nucleus,Other locations:protein complex
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Cell Biology; Metabolism
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.