Synonyms:cdk8,CDK8 protein kinase,CDK8,Cell Division Kinase 8,Cell division protein kinase 8,Cyclin Dependent kinase 8,Cyclin-dependent kinase 8,K35,Mediator complex subunit cdk8,Mediator of RNA polymerase II transcription subunit cdk8,Protein kinase K35
|Synonyms||cdk8,CDK8 protein kinase,CDK8,Cell Division Kinase 8,Cell division protein kinase 8,Cyclin Dependent kinase 8,Cyclin-dependent kinase 8,K35,Mediator complex subunit cdk8,Mediator of RNA polymerase II transcription subunit cdk8,Protein kinase K35|
|Immunogen||Synthesized peptide derived from the N-terminal region of human Cdk8|
|Recommended dilution||WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000|
|Storage instructions||Store at -20℃. Avoid freeze / thaw cycles.|
|Storage buffer||PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4|
|Background||Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.|
Western Blot analysis of 293 cells using Cdk8 Polyclonal Antibody at dilution of 1:500.