|CDK1 Polyclonal Antibody||20μL||WB,IF,ELISA||H,M,R||Rabbit|
|CDK2 Polyclonal Antibody||20μL||WB,IHC-p,IF,ELISA||H,M,R||Rabbit|
|CDK4 Polyclonal Antibody||20μL||WB,IHC-p,IF,ELISA||H,M,R||Rabbit|
|CDK6 Polyclonal Antibody||20μL||WB,IHC,ELISA||H,M||Rabbit|
|CDK7 Polyclonal Antibody||20μL||WB,IHC-p,ELISA||H,M||Rabbit|
|CDK9 Polyclonal Antibody||20μL||WB,ELISA||H,M,R||Rabbit|
|Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated)||120μL||WB,ELISA||Goat|
Western Blot analysis of K562 cells using CDK1 Polyclonal Antibody at dilution of 1:2000.
Western Blot analysis of various cells using Cdk2 Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of MCF7, K562 cells with Cdk4 Polyclonal Antibody.
Western Blot analysis of Jurkat cell using CDK6 Polyclonal Antibody at dilution of 1:150
Western Blot analysis of 293 cells with Cdk7 Polyclonal Antibody.
Western Blot analysis of 823 and Hela cell using CDK9 Polyclonal Antibody at dilution of 1:300
The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.
Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1. Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity. The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT. CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1. CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events.