Cell Counting Kit 8 (WST-8 / CCK8)[361]

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      Catalog number:E-CK-A361

      Size:
      • 100T
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      Price: $36

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      Introduction

      Elabscience® Cell Counting Kit 8 (WST-8 / CCK8) is a rapid and highly sensitive kit based on WST-8, which is widely used in cell proliferation and cytotoxicity detection.


      Detection principle

      WST-8 is a compound similar to MTT, which can be reduced to orange formazan by some dehydrogenase in mitochondria in the presence of electron coupling reagent. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth of color and the number of cells.


      Components


      Cat.

      Products

      100 Tests

      500 Tests

      1000 Tests

      Storage

      E-CK-A361

      CCK-8 Buffer

      1 mL

      5 mL

      10 mL

      -20°C

      Manual

       One Copy


       

       

      Storage

      Store at 2~8°C for one year in dark. For long time storage, please store at -20°C and the shelf life can be two year.


       

      Cautions

      1.   For long time storage, please store at -20°C. For ordinary usage, please store at 2~8°C. Avoid freeze / thaw cycles.

      2.  Mixing the cell suspension before adding to the wells to avoid cell precipitation leading to different cell number in each well.

      3.  The optimum reaction time of CCK-8 depends on the specific optimum time of colour development. It is suggested that the optimum cell number and incubation time of CCK-8 should be explored by pre-experiment CCK-8.

      4.  The detection of this kit depends on dehydrogenase-catalyzed reactions, so reductants (such as some antioxidants) may interfere with the detection. If there are more reductants in the system to be detected, try to remove them.

      5.  For your safety and health, please wear the lab coat and disposable gloves before the experiments.

      Citations

      1. CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
        Journal: The FASEB Journal(2020)
        DOI: 10.1096/fj.201902910RRR
        PMID: 32862457
        Products: E-CK-A341 E-CK-A361 E-EL-M0018 E-EL-M0019 E-EL-M0032 E-EL-M0037 E-EL-M0043 E-EL-M0044 E-EL-M0046 E-EL-M0047 E-EL-M0048 E-EL-M0731 E-EL-M1273 E-EL-M2446 
        Sample type: Cell culture supernatant,Tissue homogenate
        Reactivity: Mouse
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