CHEK1 Polyclonal Antibody

Uniprot : O14757
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience
    <
    >
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience
    • polyclonal antibody-Elabscience

      Catalog number:E-AB-30910

      Synonyms:C85740,Cell cycle checkpoint kinase,Checkpoint,S. pombe,homolog of,1,Checkpoint kinase 1,Checkpoint kinase 1 homolog (S. pombe),CHEK 1,Chek1,Chk 1,Chk1,CHK1 checkpoint homolog (S. pombe),CHK1,EC 2.7.11.1,rad27,Serine/threonine protein kinase Chk1,Serine/threonine-protein kinase CHK1,STT3,subunit of the oligosaccharyltransferase complex,homolog A (S. cerevisiae)

      Size:
      • 20 μL
      • 60 μL
      • 120 μL
      • 200 μL
      Qty:
      - +
      Price: $69

      Host: Rabbit

      Reactivity: H

      Applications: WB,IHC-p,IF,ELISA

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual
      Hot !
      • CHEK1 Polyclonal Antibody20μL
      • Phospho-CHEK1 (Ser280) Polyc...20μL
      • Phospho-CHEK1 (Ser317) Polyc...20μL
      • Phospho-CHEK1 (Ser301) Polyc...20μL
      • CHEK2 Polyclonal Antibody20μL
      • Phospho-CHEK2 (Ser516) Polyc...20μL
      • Phospho-CHEK2 (Thr68) Polycl...20μL
      • Phospho-CHEK2 (Thr383) Polyc...20μL
      • Phospho-CHEK2 (Thr387) Polyc...20μL
      • Goat Anti-Rabbit IgG (H+L)(p...120μL

      Overview

      Synonyms C85740,Cell cycle checkpoint kinase,Checkpoint,S. pombe,homolog of,1,Checkpoint kinase 1,Checkpoint kinase 1 homolog (S. pombe),CHEK 1,Chek1,Chk 1,Chk1,CHK1 checkpoint homolog (S. pombe),CHK1,EC 2.7.11.1,rad27,Serine/threonine protein kinase Chk1,Serine/threonine-protein kinase CHK1,STT3,subunit of the oligosaccharyltransferase complex,homolog A (S. cerevisiae)
      Swissprot O14757
      Source Rabbit
      Reactivity Human
      Immunogen Synthesized peptide derived from human Chk1 around the non-phosphorylation site of Ser286.
      Application WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF,ELISA
      Recommended dilution WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
      Concentration 1 mg/mL
      Clonality Polyclonal

      Properties

      Cellular localization Cytoskeleton,centrosome,Cytosol,Extracellular region or secreted,extracellular space,Nucleus,condensed nuclear chromosome,nucleoplasm,nucleus,Other locations:chromatin,cytoplasm,intracellular membrane-bounded organelle,protein complex
      Tissue specificity Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
      Isotype IgG
      Purification Affinity purification
      Conjugation Unconjugated
      Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
      Storage buffer PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
      Background Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

      Images

      Western Blot analysis of K562 cells with CHEK1 Polyclonal Antibody

      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought