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Cat.No.:E-BC-K189-M
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Synonyms | Cl |
Sample type | Serum, plasma, animal tissue |
Sensitivity | 1 mmol/L |
Detection range | 1.0-60 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 15 min |
Precision | Average inter-assay CV: 6.400%Average intra-assay CV: 3.600% |
Other instruments required | Micropipettor, Tubes, Vortex mixer, Incubator, Centrifuge. |
Storage | 2-8℃ |
Valid period | 12 months |
The chloride concentration in supernatant was significantly increased in HG group and reduced in IAA94 treated group. (**P<0.01 vs. CON, ##P<0.01 vs. HG)
SH Li et al investigate the diabetic cardiomyopathy induced by sleeve gastrectomy.. Chloride (Cl) concentration in cell supernatant was determined using chloride colorimetric assay kit (E-BC-K189-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.0-60 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
10% Mouse liver tissue homogenate |
2-3 |
10% Mouse kidney tissue homogenate |
2-3 |
10% Rat spleen tissue homogenate |
2-3 |
Human serum |
5-10 |
Mouse serum |
5-10 |
Cynomolgus monkey serum |
5-10 |
Note: The diluent is double distilled water.
DOI: 10.1155/2022/4608914 |
|
PMID: 35498125 |
Sample: H9c2 cell |