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Cholinesterase (CHE) Activity Assay Kit

Cat:E-BC-K052-S
Manual MSDS

Price: $ 260

Price: $ 180

Size:
100Assays 50Assays
Quantity:
  • Sample Type: whole blood;serum;plasma;tissue;cells
  • Detection Instrument: Spectrophotometer(520 nm)
  • Reasearch Areas: Liver Renal function biomarkers
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Product Details
Detection Principle Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with basic hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.
Synonyms CHE
Sample Type whole blood,serum,plasma,tissue,cells
Sensitivity 1.17 U/mL
Detection Range 1.17-160 U/mL
Detection Method Colorimetric method
Assay Time 60 min
Precision inter-assay CV: 9.4% | intra-assay CV: 3.7%
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 2-3
Human plasma 2-3
Mouse serum 2-3
Mouse plasma 2-3
SH-SY5Y cells 1
10% Mouse brain tissue homogenate 1
10% Rat brain tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Mouse liver tissue homogenate 1
The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.