CIM (Cimaterol) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E026

      Size:
      • 96T
      Qty:
      - +
      Price: $240

      sample_type: Urine,Tissue,serum

      Sensitivity: 0.2 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Cimaterol (CIM) in samples, such as tissue, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, CIM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CIM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of CIM. The concentration of CIM in the samples can be calculated by comparing the OD of the samples to the standard curve.



      Technical indicator

      Sensitivity: 0.2 ppb (ng/mL)

      Reaction mode: 25, 30 min~15 min

      Detection limit: Urine ---1.5 ppb; Tissue (method 1) ---2 ppb; Tissue (method 2) ---0.5 ppb; Serum---2 ppb.

      Cross-reactivity: Cimaterol---100%, Terbutaline---4%, Clenbuterol---40%, Salbutamol--8%, Brombuterol---50%, 

                                    Tulobuterol ---15%, Clorprenaline—5%

      Sample recovery rate: 90%±30%.


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

       1mL each

      (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb)

      HRP Conjugate

      6 mL

      Antibody Working Solution

      6 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer 

      25 mL

      Reconstitution Buffer

      60 mL

      Serum Diluent
      60 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other materials required but not supplied

      Instruments: Microtiter plate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: NaOH, Ethyl acetate, Acetonitrile, N-hexane, Trichloroacetic acid.


      Storage and expiry date

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.

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