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CK-16 Polyclonal Antibody

Cat:E-AB-12323
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human ;Mouse ;Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Hela
Verified Samples in IHC:Human colon cancer,Human liver cancer
Dilution

WB 1:500-1:2000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human KRT16
Abbre CK-16
Synonyms CK 16;CK-16;CK16;Cytokeratin-16;Cytokeratin16;FNEPPK;Focal non epidermolytic palmoplantar keratoderma;K 16;K16;K1C16;K1CP;Keratin 1 type I;Keratin 16;Keratin;Keratin type I cytoskeletal 16;Keratin-16;Keratin16;KRT 16;Krt16;KRT16A;NEPPK;PC1;type I cytoskeletal 16
Swissprot
Calculated MW 51kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm
Concentration 0.3 mg/mL
Buffer PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Purification Method Affinity purification
Research Areas Cancer; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.