CLE (Clenbuterol) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E014

      Size:
      • 96T
      Qty:
      - +
      Price: $138

      Sensitivity: 0.10 ppb

      Sample type: Urine, Tissue, Feed, Serum

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Clenbuterol (CLE) in urine, tissues and feed. This kit is composed of ELISA Microtiter plate pre-coated with coupled antigen, HRP conjugate, antibody, standard and other supplementary reagents. During the detection, after adding standard or sample solution, CLE in the samples competes with pre-coated coupled antigen on the ELISA Microtiter plate for CLE antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of CLE. The residual quantity of CLE in the samples can be calculated by comparing the OD of the samples to the standard curve.

       

      Technical indicator

      Sensitivity: 0.1 ppb (ng/mL)

      Reaction mode: 25, 30min~15min

      Detection limit: Urine ---0.1 ppb, Tissue (treatment method 1) ---0.4 ppb, Tissue (treatment method 2) ---0.1 ppb, Feed---1 ppb.

      Cross-reactivity: Clenbuterol ---100%, Arubendol---<1%, Mabuterol---<1%, Brombuterol---<1%, Albuterol---<1%, Ractopamine---<1%.

      Sample recovery rate: Urine ---95%±10%, Tissue, Feed---85%±15%.

       

      Kits components


      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid 

       1mL each

      (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)

      High Concentrated Standard (100 ppb)

      1 mL

      HRP Conjugate

      5.5 mL

      Antibody Working Solution

      9 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      10×Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer,Nitrogen Evaporators /Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: NaOH, Acetic ether, Concentrated HCl, Acetonitrile, Methanol, N-hexane, Anhydrous sodium sulfate.


      Storage and valid period

      Storage: Store at 2-8. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration date is on the packing box.



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