Intended Use
This kit
contains essential reagents for the development of COVID-19 IgG/IgM Lateral Flow
(Rapid Test) assay to help with the qualitative detection of IgG and IgM
antibodies specific to SARS-CoV-2 in human biological fluids.
Kit Components & Storage
The components listed can be purchased separately.
Keep the components according to the specific storage
conditions once you receive the kit.
Test principle
We have materials for development of COVID-19 IgG/IgM
Rapid Test. It applies the GICA(Gold Immunochromatography) method for the
detection of IgG and IgM specific to SARS-CoV-2 (2019-nCoV) in human bilogical
fluids. It consists of two test lines, an IgG line and an IgM line, and a
control line. The mouse anti-human IgG monoclonal is pre-coated in IgG test
line region while the mouse anti-human IgM monoclonal is pre-coated in IgM test
line region. And the goat anti-chicken IgY polyclonal is pre-coated in the
control line region. During testing, the sample reacts with SARS-CoV-2
(2019-nCoV) antigen-coated particles in the test cassette. The mixture then
migrates upward on the membrane chromatographically by capillary action and
reacts with the anti-human IgG in IgG test line region. If the sample contains
IgG antibodies to SARS-CoV-2 (2019-nCoV), a colored line will appear in IgG
test line region. In the same way, if the sample contains IgM antibodies to
SARS-CoV-2 (2019-nCoV), a colored line will appear in IgM test line region. And
if the tested sample does not contain antibodies specific to SARS-CoV-2
(2019-nCoV), no colored line will appear in either of the two test line
regions, which indicates a negative result.
To serve as a procedural control, a colored line
will always appear in the control line region, indicating that the proper
volume of sample has been added and membrane wicking has occurred.
Protocols for use
Coating on test lines and control line
Note: Prepare the required nitrocellulose filters and other related materials before this process.
C line (Control line): Dilute the Control antibody to 0.2-0.5mg/mL with Buffer, then use it for the control
line coating.
T1 line (IgG test line): Dilute the Mouse anti-human IgG monoclonal to 1.0-1.5mg/mL with Buffer, then use
it for the IgG test line coating.
T2 line (IgM test line):
Dilute the Mouse anti-human IgM
monoclonal to 0.4-0.8mg/mL with Buffer,
then use it for the IgM test line coating.
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