COVID-19 IgG/IgM Rapid Test Development Kit

    • COVID-19 IgG/IgM Rapid Test Development Kit-Elabscience
    • COVID-19 IgG/IgM Rapid Test Development Kit-Elabscience
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    • COVID-19 IgG/IgM Rapid Test Development Kit-Elabscience
    • COVID-19 IgG/IgM Rapid Test Development Kit-Elabscience
    • COVID-19 IgG/IgM Rapid Test Development Kit-Elabscience
    • COVID-19 IgG/IgM Rapid Test Development Kit-Elabscience

      Catalog number:E-ELC-RTD001

      Size:
      • 100,000 Tests
      • 500,000 Tests
      • 1,000,000 Tests
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      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Intended Use

      This kit contains essential reagents for the development of COVID-19 IgG/IgM Lateral Flow (Rapid Test) assay to help with the qualitative detection of IgG and IgM antibodies specific to SARS-CoV-2 in human biological fluids.

       

      Kit Components & Storage

      The components listed can be purchased separately.

      Keep the components according to the specific storage conditions once you receive the kit.

      Cat NO.

      Description

      Storage conditions

      E-ELC-002

      Recombinant SARS-CoV-2 N Protein (with colloidal gold conjugated)

      2-8℃ for 2 months

      E-ELC-003

      Mouse anti-human IgG monoclonal

      -20℃ for 12 months. Avoid repeated freeze-thaw cycles

      E-ELC-004

      Mouse anti-human IgM monoclonal

      -20℃ for 12 months. Avoid repeated freeze-thaw cycles

      E-ELC-005

      Goat anti-chicken IgY polyclonal

      -20℃ for 12 months. Avoid repeated freeze-thaw cycles

      E-ELC-006

      Chicken IgY (with colloidal gold conjugated)

      2-8℃ for 2 months

      E-ELC-008

      Buffer for Rapid Test development(02) 

      2-8℃ for 3 months

       

      Test principle

      We have materials for development of COVID-19 IgG/IgM Rapid Test. It applies the GICA(Gold Immunochromatography) method for the detection of IgG and IgM specific to SARS-CoV-2 (2019-nCoV) in human bilogical fluids. It consists of two test lines, an IgG line and an IgM line, and a control line. The mouse anti-human IgG monoclonal is pre-coated in IgG test line region while the mouse anti-human IgM monoclonal is pre-coated in IgM test line region. And the goat anti-chicken IgY polyclonal is pre-coated in the control line region. During testing, the sample reacts with SARS-CoV-2 (2019-nCoV) antigen-coated particles in the test cassette. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the anti-human IgG in IgG test line region. If the sample contains IgG antibodies to SARS-CoV-2 (2019-nCoV), a colored line will appear in IgG test line region. In the same way, if the sample contains IgM antibodies to SARS-CoV-2 (2019-nCoV), a colored line will appear in IgM test line region. And if the tested sample does not contain antibodies specific to SARS-CoV-2 (2019-nCoV), no colored line will appear in either of the two test line regions, which indicates a negative result.

      To serve as a procedural control, a colored line will always appear in the control line region, indicating that the proper volume of sample has been added and membrane wicking has occurred.

       

      Protocols for use

      Coating on test lines and control line

      Note: Prepare the required nitrocellulose filters and other related materials before this process.

      C line (Control line): Dilute the Control antibody to 0.2-0.5mg/mL with Buffer, then use it for the control line coating.

      T1 line (IgG test line): Dilute the Mouse anti-human IgG monoclonal to 1.0-1.5mg/mL with Buffer, then use it for the IgG test line coating.

      T2 line (IgM test line): Dilute the Mouse anti-human IgM monoclonal to 0.4-0.8mg/mL with Buffer, then use it for the IgM test line coating.



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