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Cytochrome c1 Polyclonal Antibody

Cat:E-AB-40271 Citations (2)
Manual MSDS
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Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:Rat heart,Mouse heart
Verified Samples in IHC:Human stomach,Human stomach
Dilution

WB 1:500-1:1000 , IHC 1:100-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant Mouse Cytochrome c1, heme protein, mitochondrial protein
Abbre Cytochrome c1
Synonyms Complex III subunit 4;Complex III subunit IV;CY1;CYC1;Cytochrome b c1 complex subunit 4;Cytochrome b-c1 complex subunit 4;Cytochrome bc1 complex subunit 4;Cytochrome c 1;Cytochrome c-1;Cytochrome c1;heme protein;mitochondrial;Ubiquinol cytochrome c reductase complex cytochrome c1 subunit;Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit;UQCR4
Swissprot
Calculated MW 35,29 kDa
Observed MW 34 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Mitochondrion inner membrane.
Concentration 1 mg/mL
Buffer PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification Method Antigen Affinity Purification
Research Areas Cancer; Metabolism; Signal Transduction; Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6.