Cytochrome c1 Polyclonal Antibody
- +2
Price: $ 380
Price: $ 230
Price: $ 125
Price: $ 65
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Rat heart,Mouse heart Verified Samples in IHC:Human stomach,Human stomach |
Dilution |
WB 1:500-1:1000 , IHC 1:100-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant Mouse Cytochrome c1, heme protein, mitochondrial protein |
Abbre | Cytochrome c1 |
Synonyms | Complex III subunit 4;Complex III subunit IV;CY1;CYC1;Cytochrome b c1 complex subunit 4;Cytochrome b-c1 complex subunit 4;Cytochrome bc1 complex subunit 4;Cytochrome c 1;Cytochrome c-1;Cytochrome c1;heme protein;mitochondrial;Ubiquinol cytochrome c reductase complex cytochrome c1 subunit;Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit;UQCR4 |
Swissprot | |
Calculated MW | 35,29 kDa |
Observed MW |
34 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Mitochondrion inner membrane. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.05% Proclin300 and 50% glycerol, pH7.4. |
Purification Method | Antigen Affinity Purification |
Research Areas | Cancer; Metabolism; Signal Transduction; Tags and Cell Markers |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. |
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Comparative Effects of Alpha- and Gamma-Tocopherol on Mitochondrial Functions in Alzheimer’s Disease In Vitro Model
IF:3.998
Journal:Scientific Reports
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Experimental and molecular docking studies of quercetin and vitamin E with diabetes-associated mitochondrial-ATPase as anti-apoptotic therapeutic strategies
Journal:Journal of Diabetes and Metabolic Disorders
DOI:10.1007/s40200-022-01132-x
PMID:36404854