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DMEM/F12 Complete Medium (20)

Cat:PM150312C
Manual MSDS

Price: Inquire

Size:
125mL×4
Quantity:
  • Exp: 3 months
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For research use only. Order now, ship in 3 days

Product Details
Ingredient Statement

With: DMEM/F12(PM150312)
0.1mg/mL Streptomycin
100U/mL Penicillin G
2mM L-Alanyl-L-Glutamine(PB180419)
Other

Matters Need Attention 1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.;
4. It is not suitable for long time storage at room temperature.;
5. This product is a ready-to-use medium. If there is no special need, don’t add serum, penicillin and streptomycin. It can be used directly.
Concentration Ready-to-use
Volume 125mL×4
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin content <3
Storage Condition This product can be stored at 2-8℃ for 3 months with shading light.
Shipping Condition Ice bag
  • Q1:Does this basal medium need to be filter-sterilized?

    If the medium is in powder form, it needs to be filter-sterilized after preparation. If the medium is in liquid form, there’s no need to filter-sterilize. All our liquid basal media are filtered by 0.1μm filter membrane to ensure sterilization. Additional filtration will increase the risk of contamination.

  • Q2:Why can most primary cells be cultured with poly-L-lysine (PLL)-coated vessels to promote cell attachment?

    The properties of the surface of the culture vessel are very important for cell culture. The glycoprotein (negative) on the cell surface is easy to adsorb on the hydrophilic surface. PLL has a hydrophilic surface. Polycations will interact with the anions on the cells to produce a strong adhesive force, which can prevent the cells from stacking and help the cells to adhere well.

  • Q3:Does the antibiotic need to be filtered for bacteria removal?

    All the liquid reagents sealed in bottles are filtered by 0.1μm filter membrane to ensure sterilization. Additional filtration will increase the risk of contamination.

  • Q4:Is it necessary to shade the light for storage regarding this cell-specific complete medium?

    It's quite necessary to store the complete medium shading from light, although weak scattered light does not affect the product much.

  • Q5:Can I replace the basal medium previously used with this new basal medium?

    Firstly, please check the formula of both media and check whether there are special requirements for the medium. It is replaceable when the 2 basal media are the same type. It is recommended to mix the new basal medium with the previous basal medium at 1:1 and use it to culture cells for about 1 week, then replace all with the new basal medium after the cells are in stable status. During this period, pay attention to the observation of the cell state. It is not recommended to change when the basal media are different types. If you insist on it, it’s suggested to take a small number of cells and test it in subculture for 2 to 3 passages. Observe the cell status. If there is no status change and the cells adapt well, you can replace the medium. Before replacing, it is recommended to cryopreserve a sufficient amount of cells, in case of a cell shortage due to the non-adaptation.

  • Q6:What is the reason for the color change of unopened complete medium when well stored?

    It may be caused by contamination. Test method: use a pipette to absorb the appropriate amount of complete medium, add it to the corresponding culture vessel, and then observe if any turbidity occurred after 37 ℃ incubation for about 3 days. Another reason may be air leakage from the cap. The air leakage does not necessarily lead to contamination. If no liquid leakage, it can still work. In this case, slightly loosen the cap and put it in the CO2 incubator. Generally, the color will restore. If the color has already changed when it’s just received and unopened, please contact us for a replacement.

  • Q7:What are the methods for trypsin thawing and re-warming?

    Method of trypsin thawing: thawing at 2℃ ~ 8℃, fully mixing before use. Remember to avoid repeated freeze-thaw, and it is recommended to aliquot and freeze storage in advance when the use amount is small each time. Method of trypsin re-warming: after the trypsin is dissolved, place it at room temperature for more than half an hour (the specific time depending on the volume of trypsin and room temperature); re-warming finishes when no condensed water appears on the bottle wall and it feels not cold.

  • Q8:What is the reason for the precipitation of unopened complete medium?

    The complete medium is rich in nutrients. It may be serum precipitation or other protein precipitation. It may be caused by contamination. Test method: use a pipette to absorb the appropriate amount of complete medium, and add it to the corresponding culture vessel. Observe if any turbidity occurred after 37 ℃ incubation for about 3 days. If the case of contamination is excluded, remove the precipitation by centrifugation, add a small number of cells into the complete medium, subculture for 2~3 passages, and then observe whether there is a change in the state of the cells.

  • Q9:Does this basal medium contain NaHCO3?

    You can find the formula information of this medium through our website. If no relevant information is available, please contact our technical support. Most of the basal media contain NaHCO3, except for the L-15 medium, which adopts a phosphate salt buffer system. The content of NaHCO3 in each basal medium is not necessarily the same.

  • Q10:Why the dissociation time is different on a same type of cell when the trypsin is at the same concentration?

    Firstly, the time for cell adhering and proliferation is different. Additionally, the brand of trypsin, the time after trypsin opening, and the times of freeze-thaw will also affect the dissociation effect. So the recommended dissociation time can only be used as a reference, and the determinative factor we should focus on is the cell state during the process of dissociation.