E2(Estradiol) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0065

      • 96T
      • 24T
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 40.00~1500 pg/mL

      Sensitivity: 25.00 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Goat Anti-Rabbit IgG, make solid-phase secondary antibody. And then add samples, horseradish peroxidase-labeled Estradiol and anti- Estradiol antibody, so as to form a coated secondary antibody - anti- Estradiol antibody - HRP-labeled Estradiol complex. The amount of bound labeled Estradiol is inversely proportional to that of Estradiol in the samples. The TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Estradiol in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type




      Assay time




      Detection Method


      Detection Range

      40—1500 pg/mL


      25 pg/mL

      Sample Volume


      Sample Type

      Serum, plasma and other biological fluids


       No significant cross-reactivity or interference with progesterone, estriol.

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received




      Micro ELISA Plate(Dismountable)

      8 wells ×12 strips


      Reference Standard (6 tubes)

      0.5mL /tube


      Detection Ab

      1vial  6mL


      HRP-labeled Estradiol

      1vial  6mL

      4(shading light)

      Concentrated Wash Buffer (20×)

      1vial  15mL


      Substrate Reagent A

      1vial  7mL

      4(shading light)

      Substrate Reagent B

      1vial  7mL

      4(shading light)

      Stop Solution

      1vial  7mL


      Plate Sealer



      Product Description

      1 copy


      Certificate of Analysis

      1 copy


      #: A set of Standard concentrations is 0 pg/mL, 40 pg/mL, 120 pg/mL, 250 pg/mL, 800 pg/mL, 1500 pg/mL.

      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

      Other supplies required

      Microplate reader with 450nm wavelength filter

      High-precision transferpettor, EP tubes and disposable pipette tips

      37 Incubator

      Deionized or distilled water

      Absorbent paper

      Loading slot for Wash Buffer


      1. Publication: Sun W, Wang Y Q, Yan Q, et al. Effects Of Er-Zhi-Wan On Microarchitecture And Regulation Of Wnt/β-Catenin Signaling Pathway In Alveolar Bone Of Ovariectomized Rats[J]. Journal of Huazhong University of Science and Technology-Medical Sciences, 2014, 34(1): 114-119.
        Sample Type: Serum
      2. Publication: Yan W J, Mu Y, Yu N, et al. Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet[J]. Journal of Assisted Reproduction & Genetics, 2015, 32(7): 1097-1104.
        Sample Type: Serum
      3. Publication: Zheng X, Wu G, Nie Y, et al. Electroacupuncture At The Governor Vessel And Bladder Meridian Acupoints Improves Postmenopausal Osteoporosis Through Osteoprotegerin/RANKL/RANK And Wnt/β-Catenin Signaling Pathways[J]. Experimental & Therapeutic Medicine, 2015, 10: 541-548.
        Sample Type: Serum
      4. Publication: Li J, Chang J, Li W, et al. Disruption Of Sex-Hormone Levels And Steroidogenic-Related Gene Expression On Mongolia Racerunner (Eremias Argus) After Exposure To Triadimefon And Its Enantiomers[J]. Chemosphere, 2017, 171: 554-563.
        Sample Type: Serum
      5. Publication: Yang M, Yan W J, Yin T L, et al. Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction[J]. Molecular Medicine Reports, 2016, 14(4): 3588-3594.
        Sample Type: Serum
      6. Publication: Cui X R, Long C L, Tian J, et al. Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR[J]. Cellular Physiology and Biochemistry, 2017, 41: 598-608.
        Sample Type: Serum
      7. Publication: Mu Y, Yan W J, Yin T L, et al. Curcumin Ameliorates High‑Fat Diet‑Induced Spermatogenesis Dysfunction[J]. Molecular Medicine Reports, 2016, 14(4): 3588-3594.
        Sample Type: Serum
      8. Publication: Mu Y, Yan W, Yin T, et al. Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy[J]. Scientific Reports, 2017.
        Sample Type: Serum
      9. Publication: Zheng Q, Li Y L, Zhang D D, et al. ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats[J]. Cell Death & Disease, 2017.
        Sample Type: Serum
      10. Publication: Aahmad S N S, Nourollahi S, Kazerouni F, et al. Investigation Of The Relation Between Bone Mass Density And Serum Preptin Levels In Pre-And Postmenopausal Women[J]. Journal of Bone And Mineral Metabolism, 2017.
        Sample Type: Serum
      11. Publication: Kong L H, Wang Q E, Jin J W, et al. Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells[J]. Plos One, 2017.
        Sample Type: culture supernatant
      12. Publication: Yang C, Ye J, Liu Y, et al. Methylation pattern variation between goats and rats during the onset of puberty[J]. Reproduction in Domestic Animals, 2018.
      13. Publication: Fang Y Y, Zeng P, Qu N, et al. Evidence of altered depression and dementia‐related proteins in the brains of young rats after ovariectomy[J]. Journal of Neurochemistry, 2018.
        Sample Type: Rat
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      • Q&A (1)

      D*****aSubmitted [ Feb 01 2019 ]

      Asked: what is reactivity for this E2 (estradiol) kit catalog number E-EL-0065 ? for rat, mouse, or human? thank you


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