EAH Focurose 6FF

    • EAH-6FF-Elabscience
    <
    >
    • EAH-6FF-Elabscience
    • EAH-6FF-Elabscience

      Catalog number:E-CM-AF14

      Size:
      • 25mL
      • 100mL
      • 300mL
      • 500mL
      • 1L
      Qty:
      - +
      Price: Inquire

      Matrix: Highly cross-linked 6% agarose

      Particle size range: 45-165 µm

      Average particle size: 90 µm

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Product introduction

      EAH-6FF is a kind of fast flow purification media which was prepared by covalently coupling the ethylenediamine to agarose matrix. It contains an amino group at the end of the spacer, and is mainly suitable for coupling of small molecular compounds (ligand) containing free carboxyl.

      The coupling should be reacted under acidic conditions (acid catalysis). Addition of coupling reagent (carbodiimide, such as EDC, CMC) can promote the condensation of free amino and free carboxyl. 


      Advantages

      1. Wide application range: It can be used for coupling of macro biomolecules containing amino groups.

      2. Multi-point coupling. Simple, flexible, fast and effective. It can efficiently maintain the biological activity and stability of biomolecules.

      3. Fast flow rate, high yields and easy to be implemented.


      Performance index

      Matrix

      Highly cross-linked 6% agarose

      Particle size range

      45-165 µm

      Average particle size

      90 µm

      Active group

      Amino

      Ligand density*

      0.4-0.5 mM Cl-/mL (media)

      Spacer arm

      15 atoms

      Coupling temperature

      4-25

      Coupling pH

      4.5-6.5

      pH stability

      3-14

      Chemical stability

      All commonly used buffers

      Flow rate

      250-600 cm/h

      (0.3MPa, XK16/40. Column bed height: 30 cm)

      Storage buffer

       20% Ethanol containing 0.9% NaCl

      Storage temperature

      4~8


      *: The ligand density depends on the type of ligand and coupling condition.

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