Alternate Names	Integrin alpha-4,ITGA4,CD49 antigen-like family member D,Integrin alpha-IV,VLA-4 subunit alpha,CD49d
Swissprot	P13612
References	1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4). 2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.

Product Details

Form	Liquid
Clone No.	BU49(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	Elab Fluor^® 647 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792M]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 647
	Elab Fluor^® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Human CD49d Antibody[BU49] E-AB-F1040C	20Tests, 100Tests, 100Tests×2	Check More

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