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Elab Fluor^® 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]

Uniprot : P35461,P0CW03

Cat.No.:E-AB-F1120UM
Applications: FCM
Isotype: Rat IgG2b, κ
Host: Rat

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Antigen Details

Alternate Names	Gr-1,Gr1,Ly-6G/Ly-6C,Ly6G/Ly6C
Swissprot	P35461,P0CW03
References	1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. 2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. 3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Product Details

Form	Liquid
Clone No.	RB6-8C5(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	Elab Fluor^® 647 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843M]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 647
	Elab Fluor^® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F11200	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120A	25μg, 100μg	Check More
Biotin Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120B	25μg, 100μg	Check More
FITC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120E	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120J	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UC	25μg, 100μg	Check More
PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UD	25μg, 100μg	Check More
APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UE	25μg, 100μg	Check More
PerCP Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UG	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UI	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5] E-AB-F1120UJ	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More

Citations

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