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Elab Fluor^® Violet 450 Anti-Human/Mouse CD44 Antibody[IM7]

Uniprot : P15379,P16070

Cat.No.:E-AB-F1100Q
Applications: FCM
Isotype: Rat IgG2b, κ
Host: Rat

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Antigen Details

Alternate Names	CD44 antigen,CD44,CDw44,Epican,Phagocytic glycoprotein 1,PGP-1,Phagocytic glycoprotein I,PGP-I,CD44,LHR, MDU2, MDU3, MIC4
Swissprot	P15379,P16070
Gene ID	12505,960
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Haynes BF, et al. 1991. Cancer Cells 3:347. 3. Goldstein LA, et al. 1989. Cell 56:1063. 4. Mikecz K, et al. 1995. Nat. Med. 1:558. 5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134. 6. Liu T, et al. 2009. Biol. Direct 4:40.

Product Details

Form	Liquid
Clone No.	IM7(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	Elab Fluor^® Violet 450 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842Q]
Reactivity	Human,Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® Violet 450
	Elab Fluor^® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human/Mouse CD44 Antibody[IM7]	50μg, 500μg, 1mg	Check More
Purified Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
Biotin Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
FITC Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human/Mouse CD44 Antibody[IM7]	20Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
PE Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
APC Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Human/Mouse CD44 Antibody[IM7]	25μg, 100μg	Check More

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