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FCGRT Polyclonal Antibody

Cat:E-AB-17954
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:Human placenta
Verified Samples in IHC:Human liver cancer,Human thyroid cancer
Dilution

WB 1:500-1:2000, IHC 1:30-1:150

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human FCGRT
Abbre FCGRT
Synonyms Alpha chain;Fc fragment of IgG; receptor transporter; alpha;FCGRN_HUMAN;FCGRT;FcRn alpha chain;FcRn;FCRN; alpha chain;IgG Fc fragment receptor transporter alpha chain;IgG Gc receptor;IgG receptor FcRn large subunit p51;IgG receptor FcRn large subunit p51 precursor;Immunoglobulin receptor; intestinal; heavy chain;Neonatal Fc receptor
Swissprot
Calculated MW 40 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane
Concentration 1.26 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Immunology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.