Alternate Names	Interleukin-2 receptor subunit beta,IL-2RB,p75,CD122,IL-2Rβ
Swissprot	P14784
References	1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472.

Product Details

Form	Liquid
Clone No.	TU27(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD122 is a 70-75 kD type I transmembrane glycoprotein and member of the Ig superfamily. It is IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. CD122 is constitutively expressed by NK cells and at lower levels by a subset of T cells. Its expression is upregulated upon activation. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
PE Anti-Human CD122/IL-2RB Antibody[TU27]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD122/IL-2RB Antibody[TU27]	20Tests, 100Tests, 100Tests×2	Check More

Citations

Reviews/Q&A

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Reviews
Q&A

c*********mSubmitted [ Mar 07 2024 ]

Asked: Hello, I would like to perform FACS with this antibody. Is it possible to stain for the antibody if IL2 or IL15 cytokines are bound to the sample? I am interested in assessing the surface expression of CD122 on the cells I cultivate, and these cells require IL2 for growth. If staining is not possible under these conditions, how should I prepare the sample?

adminSubmitted [ Mar 07 2024 ]

Answered: If you mean that you adding IL2 or IL15 cytokines as treatment，E-AB-F1151C is OK.

c*********mSubmitted [ Jul 06 2023 ]

Asked: Hi, this is customer I bought this antibody last week ago, and i have one question. What is the concentration of this antibody? cat number of antibody is E-AB-F1151C thank you

adminSubmitted [ Jul 06 2023 ]

Answered: Hello, Thanks for your question. The concentration of the “Test package" is internal confidential and cannot be provided. We recommend that you add 1 Test (5 μL) of antibody according to a tube of cell sample {100 μL of cell suspension (1x10^6 cells)}.

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