Foot and Mouth Disease Virus Type O Antibodies ELISA Kit

    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
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    >
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience

      Catalog number:E-AD-E017

      Size:
      • 96T
      Qty:
      - +
      Price: $277

      Reactivity: Swine , Cattle, Goat, Sheep

      Sample type: Serum, Plasma

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This ELISA kit is consisted of Micro-plate pre-coated with FMDV-O antibody, Antibody Working Solution, HRP Conjugate, Antigen Solution and other reagents. It applies Competitive-ELISA as the method for the detection of FMDV-O antibody in cattle, goat, and porcine serum samples. Diluted samples and antigen are added into the Micro-plate and incubate. Antibodies in sample will compete with antibody pre-coated on the Micro-plate for the antigen and block the combination between the antigen and Micro-plate. Then wash and add the Antibody Working Solution to incubate. Wash and add HRP Conjugate, incubate. Then wash away the unbound HRP conjugate. Add TMB substrate to the wells, it will react with the enzyme and become blue, the shade of color is of negative correlation with the content of specific antibody in the samples. At last, end the reaction by adding Stop Solution to produce a yellow product. Measure the absorbance value of each well by using a Micro-plate Reader with 450 nm wavelength, then the presence of FDMV-O antibody can be determined.

       

      Kit components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Dilution plate
      96 wells

      Antigen Solution

      5.5 mL

      Antibody Working Solution 

      5.5 mL

      HRP Conjugate 

      5.5 mL

      20×Concentrated Wash Buffer

      40 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent

      6 mL

      Stop Solution 

      6 mL

      Positive Control 

      1 mL

      Negative Control 

      1 mL

      Plate Sealer

      3 pieces

      Sealed Bag
      1 pieces

      Manual

      1 copy

       

      Experimental instrument

      Microplate Reader with 450nm wavelength filter or dual-wavelength (450/630nm)

      High-precision transferpettor, EP tubes and disposable pipette tips

      37 incubator or water bath

      Deionized or distilled water

      Absorbent paper


      Assay procedure
      1. Number: T
      ake out the Microplate, set 2 wells for negative/positive control respectively. The unused

      ELISA Microtiter plate should be sealed as soon as possible and stored at 2~8. Double well parallel experiment is recommended for detection.
      2. Add sample: 
      Take out the Microplate, set 2 wells for negative/positive control respectively. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2~8. Double well parallel experiment is recommended for detection.

      Note: The dilution factor is 1:128 for cattle/ goat sample and 1:64 for porcine sample after adding antigen.

      3. Incubate: Gently tap the plate to ensure thorough mixing, incubate at 37℃ for 30 min.
      4. Wash: Aspirate each well and wash, repeat the process 5 times, immerse 30-60 sec each time. Wash by filling each well with wash buffer (approximately 300 μL). Complete removal of liquid at each step is essential. After the last wash, remove remained wash buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.

      5. Antibody Working Solution: Add 50 µL of Antibody Working Solution into each well (except the blank control well), and incubate at 37 for 30 min.

      6Wash: Repeat Step 4 for washing.

      7. HRP conjugate: Add 100 µL of HRP conjugate into each well (except the blank control well), and incubate at 37℃ for 30 min.

      8. Wash: Repeat step 4 for washing.
      9. Color Development: Add 50 µL of substrate reagent A and 50 µL of substrate reagent B into each well, gently tap the plate to ensure thorough mixing, incubate for 15 min at 37℃. Protect from light.
      10. Stop reaction: Add 50 µL of stop solution into each well, gently mix. 、
      11. OD Measurement: Measure the absorbance value (A-value) of each well by using a Micro-plate reader with 450 nm wavelength (or use 630 nm as reference wavelength
      ).



      Storage and Expiry date

      Store at 2~8with shading light for12 months.

      Please store the opened plate at 2~8, protect from light and moisture. The valid period is 2 months.

      Valid Period: expiration date is on the packing box.


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