FQNs (Fluoroquinolones) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E054

      Size:
      • 96T
      Qty:
      - +
      Price: $260

      sample_type: Tissue,Honey,Milk,,Eggss,Urine

      Sensitivity: 0.2 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Fluoroquinolones/Quinolones (FQNS) in samples, such as milk, tissue, honey, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, FQNS in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-FQNS antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of FQNS. The concentration of FQNS in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.2 ppb (ng/mL)

      Reaction mode: 25,45 min~15min

      Detection limit: Tissue ---0.6 ppb; Honey ---0.8 ppb; Milk---6 ppb; Milk powder---12 ppb; Eggs---6 ppb; Urine---1 ppb.

      Cross-reactivity:

      Enrofloxacin

      100%

      Norfloxacin

      174%

      Ciprofloxacin

      170%

      Lomefloxacin

      132%

      Flumequine

      126%

      Peflacine

      125%

      Danofloxacin

      110%

      Sarafloxacin

      107%

      Difloxacin

      84%

      Enoxacin

      66%

      Ofloxacin (racemate)

      58%

      Oxolinic acid

      28%

      Levofloxacin

      10%

      Marbofloxacin

      4%


      Sample recovery rate:  Tissue, Honey, Milk, Milk powder, Eggs, Urine ---85%±15%.


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1mL each

      (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb)

      HRP Conjugate

      5.5 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy



      Other materials required but not supplied

      Instrument:  Microplate reader, Printer, Homogenizer, Oscillators, Nitrogen Evaporators, Water bath,  Centrifuge, Graduated pipette, Balance (sensibility 0.01g),

      High-precision transferpettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 

      Reagents:  Anhydrous acetonitrile, N-hexane, Concentrated HCl, Dichloromethane


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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