Current Position:Home>>Kits>>Metabolism Assays>> Free Cholesterol (FC) Colorimetric Assay Kit

Free Cholesterol (FC) Colorimetric Assay Kit

Cat.No.:E-BC-K004-M
Detection instrument: Microplate reader(495-525 nm,optimum wavelength: 510 nm)

Detection method: Colorimetric method

To Purchase E-BC-K004-M

Size:

96T
48T

Price: $150

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.

Performance characteristics

Synonyms	FC
Sample type	Serum, plasma, tissue
Sensitivity	0.07 mmol/L
Detection range	0.07-24 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 5.200%Average intra-assay CV: 1.900%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-24 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	1
Human serum	1
Rat serum	1
Mouse serum	1
Rabbit serum	1
10% Rat kidney tissue homogenate	1

Note: The diluent is normal saline (0.85% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

AMERICAN JOURNAL OF PATHOLOGY (2022) IF: 5.77
Paraoxonase 1 Ameliorates Renal Lipotoxicity by Activating Lipophagy and Inhibiting Pyroptosis

DOI: 10.1016/j.ajpath.2022.07.011

PMID: 35963464
Sample: plasma
ANIMAL FEED SCIENCE AND TECHNOLOGY (2021) IF: 3.247
Coating beef tallow with calcium soap improves dietary pellet quality and promotes an effective diet-induced obese mouse model

DOI: 10.1016/j.anifeedsci.2021.115008

Sample: plasma
Vascular Health and Risk Management (2023)
The Effectiveness of Nigella sativa and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats

DOI: 10.2147/VHRM.S396295

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K004-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha