Gen(Gentamicin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    <
    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E073

      Size:
      • 96T
      Qty:
      - +
      Price: $276

      sample_type: Tissue,Milk

      Sensitivity: 0.1 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Gentamicin (Gen) in tissues, milk. This kit is composed of ELISA Microtiter plate pre-coated with coupled antigen, HRP conjugate, antibody, standard and other supplementary reagents. During the detection, after adding standard or sample solution, Gen in the samples competes with pre-coated coupled antigen on the ELISA Microtiter plate for Gen antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Gen. The concentration of Gen in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.1 ppb (ng/mL)

      Reaction mode: 25, 30 min~20 min

      Detection limit: Tissue ---5ppb, Milk ---15 ppb.                                

      Cross-reactivity: Gentamicin ---100%,

      Streptomycin/Neomycin/Kanamycin/Dihydrostreptomycin ---< 0.01%,

      Sample recovery rate: 90%±30%.



      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1.5mL each

      (0ppb, 0.1ppb, 0.2ppb, 0.4ppb, 0.8ppb, 2.4ppb)

      HRP Conjugate

      7ml

      Antibody Working Solution

      7mL

      Substrate Reagent A

      7mL

      Substrate Reagent B

      7mL

      Stop Solution

      7mL

      20×Concentrated Wash Dilution

      25mL

      4×Concentrated Sample Dilution

      25mL

      10×Concentrated Tissue Dilution

      100mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments: Microplate reader, Homogenizer, Vortex Oscillators, Centrifuge, Balance (sensibility 0.01 g).

      Micropipettor: Single-channel (20-200 μL, 100-1000 μL).

      Reagents: Trichloroacetic Acid (C2HCl3O2), NaCl.


      Storage and valid period

      Storage: Store at 2-8. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration date is on the packing box.

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