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Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)

Name: Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)
Brand: Elabscience
SKU: E-BC-K234-S
Price: 150 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(19)

Cat.No.:E-BC-K234-S
Detection instrument: Spectrophotometer(505 nm)

Detection method: Colorimetric method

Manual MSDS

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Detection principle

Glucose oxidase can catalyze the oxidation of glucose to gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide and oxidizes pigment sources to form colored substances. Measure the OD value at 505 nm and glucose content can be calculated indirectly.

Performance characteristics

Synonyms	Glu
Sample type	Serum,plasma
Sensitivity	0.05 mmol/L
Detection range	0.05-30 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 1.300%Average intra-assay CV: 1.200%
Other instruments required	Vortex mixer, Micropipettor, Incubator
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Images

No significant change of serum glucose can be observed with the change of temperature.

ZJ Zhao et al investigate the effect of ambient temperature on energy balance. Glucose (Glu) level in mouse serum was determined using glucose colorimetric assay kit (E-BC-K234-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-30 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Rat plasma	1
Mouse serum	1
Human serum	1
Human plasma	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Molecular Metabolism (2022) IF: 8.568
Effects of ambient temperatures between 5 and 35 °C on energy balance, body mass and body composition in mice

DOI: 10.1016/j.molmet.2022.101551

Sample: serum
Cell Communication and Signaling (2022) IF: 7.525
Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

DOI: 10.1186/s12964-022-00882-8

PMID: 35842652
Sample: SGC-996 cell,EH-GB1 cell,GBC-SD cell
PHARMACOLOGICAL RESEARCH (2019) IF: 7.658
Dasatinib ameliorates chronic pancreatitis induced by caerulein via anti-fibrotic and anti-inflammatory mechanism

DOI: 10.1016/j.phrs.2019.104357

PMID: 31356863
Sample: Serum
FOOD RESEARCH INTERNATIONAL (2022) IF: 6.475
Acrylamide induced glucose metabolism disorder in rats involves gut microbiota dysbiosis and changed bile acids metabolism

DOI: 10.1016/j.foodres.2022.111405

PMID: 35761659
Sample: serum
CANCER SCIENCE (2022) IF: 6.518
circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

DOI: 10.1111/cas.15351

Sample: AGS cell,HGC-27 cell
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy

DOI: 10.1155/2022/4608914

PMID: 35498125
Sample: H9c2 cell
Pharmaceuticals (2022) IF: 5.215
Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats

DOI: 10.3390/ph15111420

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway

DOI: 10.3390/ijms222413336

Sample: HepG2 cell
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Dapagliflozin attenuates steatosis in livers of high-fat diet-induced mice and oleic acid-treated L02 cells via regulating AMPK/mTOR pathway

DOI: 10.1016/j.ejphar.2021.174304

Sample: L02 cells
International Journal of Environmental Research and Public Health (2022) IF: 4.614
Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid

DOI: 10.3390/ijerph19137921

PMID: 35805580
Sample: plasma
BIOCHEMICAL PHARMACOLOGY (2020) IF: 4.96
Canagliflozin inhibits p-gp function and early autophagy and improves the sensitivity to the antitumor effect of doxorubicin

DOI: 10.1016/j.bcp.2020.113856

PMID: 32061772
Sample: Cell lysate
Frontiers in Physiology (2019) IF: 3.201
Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

DOI: 10.3389/fphys.2019.01430

PMID: 31824338
Sample: Plasma
REPRODUCTION (2020) IF: 3.206
Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

DOI: 10.1530/REP-20-0381

PMID: 33112813
Sample: testis
Bioengineered (2021) IF: 3.269
KLF9 (Kruppel Like Factor 9) induced PFKFB3 (6-Phosphofructo-2-Kinase/Fructose-2, 6-Biphosphatase 3) downregulation inhibits the proliferation, metastasis and aerobic glycolysis of cutaneous squamous cell carcinoma cells

DOI: 10.1080/21655979.2021.1980644

PMID: 34612136
Sample: A431 cell
Dose-Response (2022) IF: 2.623
Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :

DOI: 10.1177/15593258221114184

Sample: blood
Biomedical Engineering Online (2021) IF: 2.819
Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

DOI: 10.1186/s12938-021-00903-6

PMID: 34217312
Sample: Huh-7 cells
BIOSCIENCE REPORTS (2020) IF: 2.942
Dexmedetomidine alleviated neuropathic pain in dorsal root ganglion neurons by inhibition of anaerobic glycolysis activity and enhancement of ROS tolerance

DOI: 10.1042/BSR20191994

PMID: 32285913
Sample: Cell sample
Molecular Medicine Reports (2021) IF: 2.952
circ-PRKCI targets miR-1294 and miR-186-5p by downregulating FOXK1 expression to suppress glycolysis in hepatocellular carcinoma

DOI: 10.3892/mmr.2021.12103

Sample: Cell sample(HCCLM3)
Chinese Medicine (2020) IF: 2.96
Protective effects of essential oil from Fructus Alpiniae zerumbet on retinal Müller gliosis via the PPAR-γ-p-CREB signaling pathway

DOI: 10.1186/s13020-019-0283-4

PMID: 31938037
Sample: Serum,Cell culture medium

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Reviews/Q&A

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Verified Customer

a*****jSubmitted [ Jun 10 2019 ]

Application:Biochemical
Description:This assay kit\'s linearity is good,It\'s more convincing than a single point.but,the determination took a little longer than expected, combined with the linearity and price, the glucose kit is worth buying，with high performance cost ratio.

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M************lSubmitted [ Mar 07 2024 ]

Asked: Can I use this kit for plant seed sample glucose detection?

adminSubmitted [ Mar 07 2024 ]

Answered: For E-BC-K234-S kit, our laboratory has verified that plant samples such as green plants and peaches can be detected, but there are differences between different samples. Theoretically, if the glucose content in the samples is higher than the sensitivity of the kit, it can be detected. It is recommended that you choose 2-3 samples to do a pre-experiment before the formal experiment.

T*********cSubmitted [ Jul 25 2023 ]

Asked: Can this assay measure glucose concentration in cell supernatants?

adminSubmitted [ Jul 25 2023 ]

Answered: E-BC-K234-S can measure glucose concentration in cell supernatants. The following are some results (for reference only): MRC-5 cell supernatant--1.330 mmol/L HepG2 cell supernatant--1.079 mmol/L

T*****aSubmitted [ Feb 01 2023 ]

Asked: Can this assay kit be used to determine the enzyme activity of Beta glucosidase enzyme?

adminSubmitted [ Feb 01 2023 ]

Answered: Thank you for your support.Sorry,this kit can not detect Beta glucosidase enzyme.

M*****iSubmitted [ Oct 27 2022 ]

Asked: How long can I keep the enzyme working solution?

adminSubmitted [ Oct 27 2022 ]

Answered: It is recommended to prepare the fresh solution before use. It can be stored at 2-8℃ for no longer than 24 hours with shading light.

M*****iSubmitted [ Sep 23 2022 ]

Asked: How many samples can a glucose kit measure?

adminSubmitted [ Sep 23 2022 ]

Answered: A 50 assays kit can test 48 samples and A 100 assays kit can test 96 samples

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