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Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Cat:E-BC-K096-M Citations (54)
Manual MSDS

Price: $ 260

Price: $ 200

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;cells;cell culture supernatant;tissue
  • Detection Instrument: Microplate reader(400-420 nm,optimum wavelength: 412 nm)
  • Reasearch Areas: Oxidative stress;Plant Physiology Series;Ferroptosis
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Product Details
Detection Principle Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Synonyms GSH-PX
Sample Type serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 17.17 U
Detection Range 17.17-518.32 U
Detection Method Colorimetric method
Assay Time 40 min
Precision inter-assay CV: 6.2% | intra-assay CV: 2.4%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Mouse serum 3-5
Rat serum 5-8
10% Mouse brain tissue homogenate 1
10% Rat liver tissue homogenate 30-60
HepG2 cells (5 mgprot/mL) 1
10% Epipremnum aureum leaves tissue homogenate 1
10% Chinese cabbage leaves tissue homogenate 3-5
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.