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Glutathione Peroxidase (GSH-Px) Activity Assay Kit

  • Cat.No.:E-BC-K096-M

  • Detection instrument: Microplate reader(400-420 nm,optimum wavelength: 412 nm)

To Purchase E-BC-K096-M

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  • 48T
Price: $260
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Detection principle

Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

Performance characteristics

Synonyms GSH-PX
Sample type Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 17.17 U
Detection range 17.17-518.32 U
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 6.2%Average intra-assay CV: 2.4%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

3-5

Rat serum

5-8

10% Mouse brain tissue homogenate

1

10% Rat liver tissue homogenate

30-60

HepG2 cell homogenate (5 mgprot/mL)

1

10% Plant tissue homogenate

1-5

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomedicine & Pharmacotherapy (2020) IF: 4.545
    Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound

    DOI: 10.1016/j.biopha.2020.110483

    PMID: 32768965

    Sample: Plasma,Tissue homogenate
  2. Cryobiology (2020) IF: 2.283
    Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen

    DOI: 10.1016/j.cryobiol.2020.04.010

    PMID: 32437678

    Sample: Sperm
  3. in vivo (2020) IF: 1.541
    Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart

    DOI: 10.21873/invivo.12067

    PMID: 32871779

    Sample: Tissue homogenate
  4. Journal of International Pharmaceutical Research (2019)
    Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits

    PMID: 99999999

    Sample: Serum
  5. Indian Journal of Animal Research (2019) IF: 0.395
    Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study

    DOI: 10.18805/ijar.B-1157

    PMID: 99999999

    Sample: Tissue homogenate
  6. Synergy (2018)
    Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin

    DOI: 10.1016/j.synres.2018.08.001

    PMID: 99999999

    Sample: Plasma
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