Glutathione Peroxidase (GSH-Px) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K096-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $260
      Can detect 40 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(400-420 nm,optimum wavelength: 412 nm)

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      Detection principle

      Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

      Performance characteristics

      Synonyms GSH-PX
      Sample type serum,plasma,cells,cell culture supernatant,tissue
      Sensitivity 17.17 U
      Detection range 17.17-518.32 U
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 40 min
      Precision Average inter-assay CV: 6.2%Average intra-assay CV: 2.4%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      1

      Mouse serum

      3-5

      Rat serum

      5-8

      10% Mouse brain tissue homogenate

      1

      10% Rat liver tissue homogenate

      30-60

      HepG2 cell homogenate (5 mgprot/mL)

      1

      10% Plant tissue homogenate

      1-5

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      Citations

      1. Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound
        IF: 4.545
        Journal: Biomedicine & Pharmacotherapy(2020)
        DOI: 10.1016/j.biopha.2020.110483
        PMID: 32768965
        Products: E-BC-K022-M E-BC-K096-M E-EL-0070 E-EL-R3019 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Rat
      2. miR-590-3p Attenuates Acute Kidney Injury by Inhibiting Tumor Necrosis Factor Receptor-Associated Factor 6 in Septic Mice
        IF: 2.884
        Journal: Inflammation(2018)
        DOI: 10.1007/s10753-018-0921-5
        PMID: 30392071
        Products: E-BC-K025-M E-BC-K031-M E-BC-K091-M E-BC-K096-M E-BC-K210 E-BC-K235-M E-BC-K236-M E-EL-0076 
        Sample type: Serum
        Reactivity: Mouse
      3. Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen
        IF: 2.283
        Journal: Cryobiology(2020)
        DOI: 10.1016/j.cryobiol.2020.04.010
        PMID: 32437678
        Products: E-BC-K022-M E-BC-K031-M E-BC-K096-M 
        Sample type: Sperm
        Reactivity: Chicken
      4. Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart
        IF: 1.541
        Journal: in vivo(2020)
        DOI: 10.21873/invivo.12067
        PMID: 32871779
        Products: E-BC-K046-M E-BC-K096-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      5. Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits
        Journal: Journal of International Pharmaceutical Research(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K022-M E-BC-K025-M E-BC-K029-S E-BC-K030-M E-BC-K096-M 
        Sample type: Serum
        Reactivity: Rabbit
      6. Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study
        IF: 0.395
        Journal: Indian Journal of Animal Research(2019)
        DOI: 10.18805/ijar.B-1157
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-M E-BC-K030-M E-BC-K031-M E-BC-K096-M 
        Sample type: Tissue homogenate
        Reactivity: Mussel
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