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Glutathione Reductase (GR) Activity Assay Kit

Cat:E-BC-K099-S Citations (9)
Manual MSDS

Price: $ 200

Price: $ 150

Size:
100Assays 50Assays
Quantity:
  • Sample Type: serum;plasma;tissue;cells
  • Detection Instrument: Spectrophotometer(340 nm)
  • Reasearch Areas: Oxidative stress;Ferroptosis
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Product Details
Detection Principle With the coenzyme as hydrogen donor, GSSG can be reduced to GSH under the catalysis of GR. Then the GSH content increased and NADPH decreased. The decrease of NADPH absorbance can be measured at 340 nm. The activity of GR can be calculated by detecting the change of NADPH.
Synonyms GR
Sample Type serum,plasma,tissue,cells
Sensitivity 6.2 U/L
Detection Range 6.2-320 U/L
Detection Method Colorimetric method
Assay Time 90 min
Precision inter-assay CV: 2.5% | intra-assay CV: 2.1%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Human plasma 1
Mouse serum 1
Mouse plasma 1
Rat serum 1
Rat plasma 1
10% Mouse liver tissue homogenization 1-3
10% Epipremnum aureum tissue homogenization 1
The diluent is double distilled water or normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.