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HAND1 Polyclonal Antibody

Cat:E-AB-19541
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:Human fetal brain
Verified Samples in IHC:Human esophagus cancer,Human brain
Dilution

WB 1:500-1:2000, IHC 1:20-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human HAND1
Abbre HAND1
Synonyms autonomic nervous system and neural crest derivatives-expressed protein 1;Basic helix loop helix transcription factor HAND1;bHLHa27;Class A basic helix-loop-helix protein 27;eHAND;Extraembryonic tissues;Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1;HAND 1;HAND1;HAND1;Heart and neural crest derivatives expressed 1;Heart and neural crest derivatives expressed protein 1;heart;Heart- and neural crest derivatives-expressed protein 1;Hxt;Thing 1;Thing1 ;Thing1
Swissprot
Calculated MW 24 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus>nucleoplasm. Nucleus>nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
Concentration 0.9 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.