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Current Position:Home>>Kits>>Metabolism Assays>> High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

Citations(26)

  • Cat.No.:E-BC-K221

  • Detection instrument: Microplate reader(546 nm), Biochemistry analyzer(546 nm)

Manual MSDS

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  • 96T
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Detection principle

The generated red purple pigment have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the HDL-C content in the sample can be calculated.

Performance characteristics

Synonyms HDL-C
Sample type Serum,plasma,cells,culture supernatant,tissue
Detection range 0.065-3.8 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Storage 2-8℃
Valid period 12 months

Images

TC levels in serum was significantly decreased in OVX/HFFD/D-Gal group, and increased in perindopril treatment group (a. significant change with normal control; b. significant change with OVX/HFFD/D-Gal). No significant difference of HDL-C level can be observed in hippocampal tissue.

B A S Messiha et al found that perindopril ameliorates Alzheimer’s disease progression. High density lipoprotein cholesterol (HDL-C) levels in rat serum and hippocampus were determined using HDL-C colorimetric assay kit (E-BC-K221).

Citations

  1. Food & Function (2023) IF: 6.317
    Analysis of the mechanism of action of quercetin in the treatment of hyperlipidemia based on metabolomics and intestinal flora

    DOI: 10.1039/D2FO03509J
    Sample: serum
  2. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    N6-Methyladenosine Methyltransferase METTL3 Promotes Angiogenesis and Atherosclerosis by Upregulating the JAK2/STAT3 Pathway via m6A Reader IGF2BP1.

    DOI: 10.3389/fcell.2021.731810
    Sample: plasma
  3. CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.691
    ATGL deficiency aggravates pressure overload-triggered myocardial hypertrophic remodeling associated with the proteasome-PTEN-mTOR-autophagy pathway

    DOI: 10.1007/s10565-022-09699-0

    PMID: 35218467

    		 Sample: serum
  4. EUROPEAN JOURNAL OF PHARMACOLOGY (2023) IF: 5.195
    Pancreastatin inhibitor PSTi8 ameliorates streptozotocin-induced diabetes by suppressing hepatic glucose production

    DOI: 10.1016/j.ejphar.2023.175559
    Sample: plasma
  5. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Integrating metabolomics and network pharmacology to investigate Panax japonicus prevents kidney injury in HFD/STZ-induced diabetic mice

    DOI: 10.1016/j.jep.2022.115893
    Sample: serum
  6. MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.309
    Gymnaster Koraiensis Extract Alleviated Metabolic Syndrome Symptoms and Stimulated UCP1‐Independent Energy Consumption via AMPK Activation in White Adipose Tissue

    DOI: 10.1002/mnfr.202000490
    Sample: Serum
  7. Food Bioscience (2022) IF: 5.318
    Phytosterol of lotus seed core powder alleviates hypercholesterolemia by regulating gut microbiota in high-cholesterol diet-induced C57BL/6J mice

    DOI: 10.1016/j.fbio.2022.102279
    Sample: serum,liver tissue
  8. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: serum
  9. ACS Omega (2022) IF: 4.132
    Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

    DOI: 10.1021/acsomega.2c03619

    PMID: 35967043

    		 Sample: serum
  10. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

    DOI: 10.1007/s11356-021-14871-w

    PMID: 34216342

    		 Sample: serum
  11. TOXICOLOGY LETTERS (2021) IF: 4.374
    Alpha-lipoic acid attenuates silica-induced pulmonary fibrosis by improving mitochondrial function via AMPK/PGC1α pathway activation in C57BL/6J mice

    DOI: 10.1016/j.toxlet.2021.07.003

    PMID: 34252510

    		 Sample: serum
  12. Journal of Functional Foods (2021) IF: 4.451
    Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling

    DOI: 10.1016/j.jff.2021.104581
    Sample: blood
  13. JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    		 Sample: serum
  14. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022) IF: 3.168
    Serum Levels of Free Fatty Acids in Obese Mice and Their Associations with Routine Lipid Profiles

    DOI: 10.2147/DMSO.S348800

    PMID: 35140491

    		 Sample: serum
  15. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    		 Sample: Plasma
  16. MEAT SCIENCE (2019) IF: 3.483
    Effects of naturally-produced lovastatin on carcass characteristics, muscle physico-chemical properties and lipid oxidation and cholesterol content in goats

    DOI: 10.1016/j.meatsci.2019.04.008
    Sample: serum
  17. LIFE SCIENCES (2020) IF: 3.647
    Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

    DOI: 10.1016/j.lfs.2020.118118
    Sample: serum
  18. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
    Pancreastatin inhibitor activates AMPK pathway via GRP78 and ameliorates dexamethasone induced fatty liver disease in C57BL/6 mice

    DOI: 10.1016/j.biopha.2019.108959
    Sample: plasma
  19. Heliyon (2020) IF: 3.776
    PSTi8 with metformin ameliorates perimenopause induced steatohepatitis associated ER stress by regulating SIRT-1/SREBP-1c axis

    DOI: 10.1016/j.heliyon.2020.e05826
    Sample: plasma
  20. THERIOGENOLOGY (2020) IF: 2.094
    Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model

    DOI: 10.1016/j.theriogenology.2020.09.023

    PMID: 32980686

    		 Sample: Serum
  21. Journal of Developmental Origins of Health and Disease (2020) IF: 2.456
    Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats

    DOI: 10.1017/S2040174420000525
    Sample: plasma
  22. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    		 Sample: Plasma,Tissue homogenate
  23. International Journal of Clinical and Experimental Pathology (2018) IF: 1.396
    Pitavastatin attenuates atherosclerosis by suppressing NF-κB signaling in a high-cholesterol diet plus balloon catheter injury rabbit model

    PMID: 31938118

    		 Sample: serum
  24. TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
    Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

    DOI: 10.1177/0748233720937199
    Sample: Plasma
  25. BMC Veterinary Research (2020) IF: 1.835
    Tea polyphenols attenuate liver inflammation by modulating obesity-related genes and down-regulating COX-2 and iNOS expression in high fat-fed dogs

    DOI: 10.1186/s12917-020-02448-7

    PMID: 32641048

    		 Sample: Serum,Tissue homogenate
  26. Heliyon (2021)
    Phyllanthus amarus attenuated derangement in renal-cardiac function, redox status, lipid profile and reduced TNF-α, interleukins-2, 6 and 8 in high salt diet fed rats

    DOI: 10.1016/j.heliyon.2021.e08106
    Sample: serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

M*****iSubmitted [ Oct 18 2022 ]

Asked: The samples have been frozen at -80 degrees celsius for 4 months. Does that have a big impact on the results?

Reply

A

adminSubmitted [ Oct 18 2022 ]

Answered: Unfortunately, your sample has been frozen for a to long, the results will be affected and be unreal.

Q

M*****iSubmitted [ Sep 06 2022 ]

Asked: Will hemolysis samples have any effect on this measurement?

Reply

A

adminSubmitted [ Sep 06 2022 ]

Answered: For this index, hemolysis samples have little effect.

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