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Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

Cat:E-BC-K143 Citations (11)
MSDS

Price: $ 660

Size:
100Assays
Quantity:
  • Sample Type: serum
  • Detection Instrument: Biochemistry analyzer(340 nm), Spectrophotometer(340 nm)
  • Reasearch Areas: Amino acids proteins
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Product Details
Detection Principle Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.
Synonyms Hcy
Sample Type serum
Detection Range 0-50 μmol/L
Detection Method Colorimetric method
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months