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HSPA2 Polyclonal Antibody

Cat:E-AB-52655
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Mouse brain,Human cerebrum,NIH/3T3,A549,Jurkat,Hela,231
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Fusion protein of human HSPA2
Abbre HSPA2
Synonyms 70kDa;Hcp70.2;Heat shock 70 kDa protein 2;Heat shock protein 2;Heat shock protein 70.2;Heat shock related 70 kDa protein 2;Heat shock-related 70 kDa protein 2;Heat-shock protein;70-KD;2;Heat-shock protein;70-KD;3;HSP70 2;HSP70 3;Hsp70-2;HSP70-3;HSP70.2;HSP70A2;HSP72;HSP72;HSPA2;Hspt70;Hst70;MGC58299;MGC7795;MGC93458;OTTHUMP00000180664;Testis-specific heat shock protein-related
Swissprot
Calculated MW 70 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoskeleton,meiotic spindle,Cytosol,Extracellular region or secreted,blood microparticle,extracellular exosome,Mitochondrion,Nucleus,male germ cell Nucleus,synaptonemal complex,Plasma Membrane,CatSper complex,Other locations:cell surface,membrane,myelin sheath
Concentration 0.6 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins,encoded by the gene originally described as testis-specific. Recently,it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity,function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently,HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA4 protein in testis and multiple cell lines. (21373891)