Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) CLIA Kit

    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    <
    >
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience
    • CLIA-Elabscience

      Catalog number:E-CL-H0255

      Synonyms:A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

      Size:
      • 96T
      Qty:
      - +
      Price: $650

      Reactivity: Human

      Detection Range: 1.25~80 ng/mL

      Sensitivity: 0.75 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This CLIA kit applies to the in vitro quantitative determination of Human AMBP/α1M concentrations in serum, plasma and other biological fluids.

      Test principle

      This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AMBP/α1M. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMBP/α1M and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMBP/α1M, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AMBP/α1M. You can calculate the concentration of Human AMBP/α1M in the samples by comparing the RLU value of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 4.5h
      Reactivity Human
      Detection method Chemiluminescence
      Detection range 1.25-80 ng/mL
      Sensitivity 0.75 ng/mL
      Sample volume 100μL
      Sample type Serum, plasma and other biological fluids
      Repeatability CV < 15%

      Specificity

      This kit recognizes Human AMBP/α1M in samples. No significant cross-reactivity or interference between Human AMBP/α1M and analogues was observed.

      Typical data

      As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (ng/mL)
      RLU Average Corrected
      80 37234
      37680
      37457 37428
      40 16027
      16211
      16119 16090
      20 8117
      6783
      7450 7421
      10 3306
      3926
      3616 3587
      5 1826
      1822
      1824 1795
      2.5 984
      934
      959 930
      1.25 504
      566
      535 506
      0 28
      30
      29 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AMBP/α1M were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AMBP/α1M were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 3.92 9.19 32.67 4.24 9.60 32.28
      Standard deviation 0.42 0.96 2.57 0.41 1.09 2.69
      C V (%) 10.71 10.45 7.87 9.67 11.35 8.33

      Recovery

      The recovery of Human AMBP/α1M spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 93-110 101
      EDTA plasma (n=5) 86-100 93
      Cell culture media (n=5) 86-97 92

      Linearity

      Samples were spiked with high concentrations of Human AMBP/α1M and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 93-104 87-99 100-116
      Average (%) 98 93 108
      1:4 Range (%) 96-113 92-102 99-112
      Average (%) 104 97 105
      1:8 Range (%) 96-110 94-108 103-118
      Average (%) 103 100 109
      1:16 Range (%) 99-115 98-111 102-115
      Average (%) 106 105 108

      Kit Components& Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro CLIA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent A 1 vial, 5 mL 4℃ (shading light)
      Substrate Reagent B 1 vial, 5 mL 4℃ (shading light)
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Chemiluminescence immunoassay analyzer
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay procedure

      1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.

      2.Remove the liquid.

      3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.

      6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.

      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less

      Browsing History


        People Also Bought