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Human FNDC5(Fibronectin type Ⅲ domain-containing protein 5) ELISA Kit

  • Cat.No.:E-EL-H2254

  • Reactivity: Human

To Purchase E-EL-H2254

Size:
  • 96T
  • 24T
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human FNDC5 in samples. No significant cross-reactivity or interference between Human FNDC5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human FNDC5 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FNDC5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FNDC5. You can calculate the concentration of Human FNDC5 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FNDC5 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FNDC5 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.51 0.87 4.57 0.51 0.86 4.33
Standard deviation 0.03 0.05 0.14 0.03 0.05 0.23
CV (%) 5.88 5.75 3.06 5.88 5.81 5.31

Recovery

The recovery of Human FNDC5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 90-102 96
Cell culture media (n=8) 87-98 93

Linearity

Samples were spiked with high concentrations of Human FNDC5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q8NAU1
SynonymsFRCP2

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY (2022) IF: 2.586
    The effect of sarcopenia and serum myokines on prognosis and survival in cirrhotic patients: a multicenter cross-sectional study

    DOI: 10.1097/MEG.0000000000002461

    PMID: 36281901

    Sample: serum
  2. JOURNAL OF DIABETES AND ITS COMPLICATIONS (2021) IF: 2.852
    Effects of increased physical activity and/or weight loss diet on serum myokine and adipokine levels in overweight adults with impaired glucose metabolism

    DOI: 10.1016/j.jdiacomp.2021.107892

    PMID: 33685795

    Sample: Serum
  3. ANAIS BRASILEIROS DE DERMATOLOGIA (2020) IF: 1.121
    Serum irisin level, insulin resistance, and lipid profiles in patients with hidradenitis suppurativa: a case-control study

    DOI: 10.1016/j.abd.2020.04.009

    PMID: 33008659

    Sample: Serum
  4. Journal of Clinical Research in Pediatric Endocrinology (2019) IF: 1.285
    The Effect of Pubertal Stage on the Concentrations of the Novel Adipomyokine, Irisin, in Male Adolescents

    DOI: 10.4274/jcrpe.galenos.2019.2019.0119

    Sample: serum
  5. JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2018) IF: 0.545
    Maternal serum irisin levels in early and late-onset pre-eclamptic and healthy pregnancies

    DOI: 10.1080/01443615.2017.1399260

    Sample: serum
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