Current Position:Home>>Kits>>ELISA Kits>> Human HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
Cat.No.:E-EL-H6066
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 62.50-4000 pg/mL |
Sensitivity | 37.50 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HIF-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HIF-1α were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 177.29 | 422.96 | 1738.08 | 194.33 | 486.58 | 1980.59 |
Standard deviation | 9.41 | 20.26 | 71.09 | 10.47 | 24.09 | 91.11 |
CV (%) | 5.31 | 4.79 | 4.09 | 5.39 | 4.95 | 4.60 |
The recovery of Human HIF-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-109 | 102 |
EDTA plasma (n=8) | 99-109 | 104 |
Cell culture media (n=8) | 101-112 | 105 |
Samples were spiked with high concentrations of Human HIF-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
Q16665
|
Synonyms | HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor |
Research Area | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 33389301 |
Sample: Serum |
PMID: 34782683 |
Sample: serum |
DOI: 10.1111/jre.12973 |
|
PMID: 35037251 |
Sample: Periodontal ligament stem cell (PDLSCs) |
PMID: 35821400 |
Sample: serum |
DOI: 10.1002/jcb.25885 |
|
Sample: Cell lysate |
PMID: 32637689 |
Sample: Serum |
DOI: 10.21037/atm-21-6812 |
|
Sample: adipose-derived stem cell (ASCs) |
Sample: Cell lysate |
Sample: urine |
DOI: 10.1002/jcla.24073 |
|
PMID: 34708885 |
Sample: serum |
PMID: 34493232 |
Sample: serum |
Sample: Tissue homogenate |
Sample: Cell lysate |
Sample: serum |
Sample: Serum |
PMID: 33183306 |
Sample: Tissue homogenate |
DOI: 10.1155/2019/7656105 |
|
Sample: Serum |
Sample: serum |
Sample: Serum |
M***********rSubmitted [ Nov 28 2022 ]
Asked: Dear team, I am looking for a human HIF1a ELISA. Can you please send me an offer and the specifications? I would like to measure cell lysates of intestinal cells. They were lysed with NP-40. Is that a problem? Thanks for helping me. Kind regards Marita Meurer
Reply
adminSubmitted [ Nov 28 2022 ]
Answered: Dear Marita,we will reply to you by email. Please pay attention to the mailbox.