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Human IFN-β(Interferon Beta) ELISA Kit

Citations(27)Uniprot : P01574

  • Cat.No.:E-EL-H0085

  • Reactivity: Human
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IFN-β in samples. No significant cross-reactivity or interference between Human IFN-β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IFN-β concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFN-β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFN-β were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 105.20 289.60 951.90 107.40 261.00 1001.00
Standard deviation 5.90 14.20 40.00 5.40 11.70 54.10
CV (%) 5.61 4.90 4.20 5.03 4.48 5.40

Recovery

The recovery of Human IFN-β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 93
EDTA plasma (n=8) 92-106 98
Cell culture media (n=8) 86-98 93

Linearity

Samples were spiked with high concentrations of Human IFN-β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01574
SynonymsIFNB1, IFB, IFF, IFNB
Research AreaCancer, Cardiovascular, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Signal Transduction and Targeted Therapy (2021) IF: 18.187
    Differential immune responses in pregnant patients recovered from COVID-19

    DOI: 10.1038/s41392-021-00703-3

    PMID: 34326311

    		 Sample: blood
  2. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    		 Sample: Tissue homogenate
  3. CLINICAL IMMUNOLOGY (2023) IF: 10.19
    CD47-targeted immunotherapy unleashes antitumour immunity in Epstein-Barr virus-associated gastric cancer

    DOI: 10.1016/j.clim.2023.109238
    Sample: macrophages
  4. ONCOGENE (2021) IF: 9.8670
    Necroptotic virotherapy of oncolytic alphavirus M1 cooperated with Doxorubicin displays promising therapeutic efficacy in TNBC

    DOI: 10.1038/s41388-021-01869-4

    PMID: 34155344

    		 Sample: MDA-MB-468 cells,HCC1806 cells,MDA-MB-361 cells
  5. Cell Death & Disease (2020) IF: 8.4689
    Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion

    DOI: 10.1038/s41419-020-2665-z

    PMID: 32532953

    		 Sample: Cell culture supernatant
  6. Cell and Bioscience (2021) IF: 7.133
    β-adrenergic receptor inhibition enhances oncolytic herpes virus propagation through STAT3 activation in gastric cancer

    DOI: 10.1186/s13578-021-00687-1

    PMID: 34544479

    		 Sample: HGC-27 cell
  7. Cell Reports (2019) IF: 7.815
    The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization

    DOI: 10.1016/j.celrep.2019.01.030

    PMID: 30699354

    		 Sample: Cell culture supernatant
  8. Cell Death & Disease (2020) IF: 6.304
    Annexin-A1 promotes RIG-I-dependent signaling and apoptosis via regulation of the IRF3–IFNAR–STAT1–IFIT1 pathway in A549 lung epithelial cells

    DOI: 10.1038/s41419-020-2625-7
    Sample: Cell culture supernatant
  9. Frontiers in Immunology (2017) IF: 6.429
    The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3

    DOI: 10.3389/fimmu.2017.00779
    Sample: Cell culture supernatant
  10. Frontiers in Immunology (2017) IF: 6.429
    Influenza A Virus PA Antagonizes Interferon-β by Interacting with Interferon Regulatory Factor 3

    DOI: 10.3389/fimmu.2017.01051
    Sample: Cell culture supernatant
  11. PLoS Pathogens (2021) IF: 6.823
    JMJD6 negatively regulates cytosolic RNA induced antiviral signaling by recruiting RNF5 to promote activated IRF3 K48 ubiquitination

    DOI: 10.1371/journal.ppat.1009366

    PMID: 33684176

    		 Sample: Cell culture supernatant
  12. JOURNAL OF VIROLOGY (2021) IF: 5.103
    Peroxiredoxin 1 Interacts with TBK1/IKKε and Negatively Regulates Pseudorabies Virus Propagation by Promoting Innate Immunity | Journal of Virology

    DOI: 10.1128/JVI.00923-21
    Sample: HEK293T cell
  13. FEBS Journal (2021) IF: 5.542
    Clinically used JAK inhibitor blunts dsRNA-induced inflammation and calcification in aortic valve interstitial cells

    DOI: 10.1111/febs.16026

    PMID: 34009721

    		 Sample: VIC cells
  14. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Broadly Antiviral Activities of TAP1 through Activating the TBK1-IRF3-Mediated Type I Interferon Production

    DOI: 10.3390/ijms22094668

    PMID: 33925089

    		 Sample: Cell lysate
  15. Frontiers in Pharmacology (2022) IF: 5.988
    A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.

    DOI: 10.3389/fphar.2022.827128
    Sample: HepG2.2.15 cell
  16. Frontiers in Microbiology (2017) IF: 4.076
    miR-194 Inhibits Innate Antiviral Immunity by Targeting FGF2 in Influenza H1N1 Virus Infection

    DOI: 10.3389/fmicb.2017.02187
    Sample: Cell culture supernatant,Bronchoalveolar Lavage Fl
  17. ANTIVIRAL RESEARCH (2018) IF: 4.307
    Encephalomyocarditis virus 2C protein antagonizes interferon-β signaling pathway through interaction with MDA5

    DOI: 10.1016/j.antiviral.2018.10.010
    Sample: HEK-293A cells
  18. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2019) IF: 4.328
    Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    		 Sample: Serum
  19. JOURNAL OF VIROLOGY (2020) IF: 4.501
    Protein tyrosine phosphatase SHP2 suppresses host innate immunity against influenza A virus through regulating EGFR-mediated signaling

    DOI: 10.1128/JVI.02001-20

    PMID: 33361428

    		 Sample: Cell culture supernatant
  20. JOURNAL OF VIROLOGY (2017) IF: 4.663
    Matrix Metalloproteinase 9 Facilitates Hepatitis B Virus Replication through Binding with Type I Interferon (IFN) Receptor 1 To Repress IFN/JAK/STAT Signaling

    DOI: 10.1128/JVI.01824-16
    Sample: Cell culture supernatant
  21. JOURNAL OF IMMUNOLOGY (2017) IF: 4.856
    A Novel Function of F-Box Protein FBXO17 in Negative Regulation of Type I IFN Signaling by Recruiting PP2A for IFN Regulatory Factor 3 Deactivation

    DOI: 10.4049/jimmunol.1601009
    Sample: Cell culture supernatant
  22. VIRUS RESEARCH (2021) IF: 3.303
    microRNA-125a targets MAVS and TRAF6 to modulate interferon signaling and promote HCV infection

    DOI: 10.1016/j.virusres.2021.198336

    PMID: 33577860

    		 Sample: Cell culture supernatant
  23. Scientific Reports (2020) IF: 3.998
    Cellular uptake of extracellular nucleosomes induces innate immune responses by binding and activating cGMP-AMP synthase (cGAS)

    DOI: 10.1038/s41598-020-72393-w

    PMID: 32958884

    		 Sample: Cell culture supernatant
  24. Innate Immunity (2019) IF: 2.173
    Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways:

    DOI: 10.1177/1753425919877680
    Sample: Cell culture supernatant
  25. ARCHIVES OF VIROLOGY (2014) IF: 2.282
    Hepatitis C virus infection decreases the expression of Toll-like receptors 3 and 7 via upregulation of miR-758

    DOI: 10.1007/s00705-014-2167-3
    Sample: Cell culture supernatant
  26. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020) IF: 2.352
    Anti-inflammatory effects of nobiletin on TLR4/TRIF/IRF3 and TLR9/IRF7 signaling pathways in prostate cancer cells

    DOI: 10.1080/08923973.2020.1725040

    PMID: 32048561

    		 Sample: Cell culture supernatant
  27. INTERNATIONAL IMMUNOPHARMACOLOGY (2017) IF: 2.956
    Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways

    DOI: 10.1016/j.intimp.2017.11.009
    Sample: Cell culture supernatant
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