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Human IL-10(Interleukin 10) ELISA Kit

  • Cat.No.:E-EL-H0103

  • Reactivity: Human

To Purchase E-EL-H0103

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference between Human IL-10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-10 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-10. You can calculate the concentration of Human IL-10 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-10 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 26.20 42.70 233.00 26.00 44.20 228.10
Standard deviation 1.80 2.60 7.70 1.70 2.00 7.10
CV (%) 6.87 6.09 3.30 6.54 4.52 3.11

Recovery

The recovery of Human IL-10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 96
EDTA plasma (n=8) 83-95 90
Cell culture media (n=8) 92-105 99

Linearity

Samples were spiked with high concentrations of Human IL-10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P22301
SynonymsIL10, IL10A, CSIF, TGIF
Research AreaCancer,Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2020) IF: 4.352
    S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes

    DOI: 10.1016/j.bbadis.2020.165895

    PMID: 32681864

    Sample: Serum
  2. European Journal of Internal Medicine (2020) IF: 4.329
    Treating periodontal disease in patients with myocardial infarction: A randomized clinical trial

    DOI: 10.1016/j.ejim.2019.08.012

    PMID: 31810741

    Sample: Serum
  3. Diabetes research and clinical practice (2019) IF: 4.234
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  4. Cells (2018) IF: 4.829
    Mesenchymal Stem Cells (MSCs) Coculture Protects [Ca2+] i Orchestrated Oxidant Mediated Damage in Differentiated Neurons In Vitro

    DOI: 10.3390/cells7120250

    PMID: 30563298

    Sample: Cell culture supernatant
  5. Bioconjugate Chemistry (2016) IF: 4.818
    Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms.

    DOI: 10.1021/acs.bioconjchem.6b00600

    PMID: 28004910

    Sample: Cell culture supernatant
  6. Molecular immunology (2019) IF: 3.188
    IL-16 regulates macrophage polarization as a target gene of miR-145-3p

    DOI: 10.1016/j.molimm.2018.12.027

    PMID: 30634164

    Sample: Cell lysate
  7. Tumor Biology (2017) IF: 3.65
    Pkcζ In Prostate Cancer Cells Represses The Recruitment And M2 Polarization Of Macrophages In The Prostate Cancer Microenvironment.

    DOI: 10.1177/1010428317701442

    PMID: 28631559

    Sample: Cell culture supernatant,Cell lysate
  8. Plos Neglected Tropical Diseases (2016) IF: 3.834
    Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.

    DOI: 10.1371/journal.pntd.0004806

    PMID: 27348302

    Sample: Serum,Cell culture supernatant
  9. World Journal of Gastroenterology (2016) IF: 3.365
    Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages.

    DOI: 10.3748/wjg.v22.i17.4354

    PMID: 27158204

    Sample: Cell culture supernatant
  10. Sleep and Breathing (2020) IF: 2.162
    Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice

    DOI: 10.1007/s11325-020-02166-2

    PMID: 32840731

    Sample: Tissue homogenate
  11. Journal of Zhejiang University. Science. B (2020) IF: 2.082
    Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China

    DOI: 10.1631/jzus.B2000174

    PMID: 32425003

    Sample: Serum,Plasma
  12. Immunopharmacology and Immunotoxicology (2020) IF: 2.352
    A case of asthma with Beh?et's disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions

    DOI: 10.1080/08923973.2020.1789656

    PMID: 32605504

    Sample: Serum
  13. Neurotoxicity research (2020) IF: 2.992
    Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased pro-inflammatory Cytokines

    DOI: 10.1007/s12640-020-00193-1

    PMID: 32335809

    Sample: Serum
  14. Microbial pathogenesis (2019) IF: 2.914
    Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles

    DOI: 10.1016/j.micpath.2019.103719

    PMID: 31493501

    Sample: Cell culture supernatant
  15. Acta tropica (2019) IF: 2.509
    Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India

    DOI: 10.1016/j.actatropica.2018.10.017

    PMID: 30395811

    Sample: Serum
  16. Evidence-Based Complementary and Alternative Medicine (2019) IF: 2.064
    In Vitro Immunomodulatory Effect of Food Supplement from Aloe vera

    DOI: 10.1155/2019/5961742

    PMID: 30941196

    Sample: Cell culture supernatant
  17. International Journal of Infectious Diseases (2015) IF: 2.532
    Decreased Monocyte Subsets And TLR4-Mediated Functions In Patients With Acute Severe Fever With Thrombocytopenia Syndrome (SFTS).

    DOI: 10.1016/j.ijid.2015.12.009

    PMID: 26701820

    Sample: Cell culture supernatant
  18. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Systematically transplanted human gingiva-derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis

    DOI: 10.3892/etm.2019.8256

    PMID: 31885706

    Sample: Serum
  19. Genetic testing and molecular biomarkers (2018) IF: 1.181
    Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity

    DOI: 10.1089/gtmb.2018.0174

    PMID: 30562142

    Sample: Plasma
  20. International Journal of Clinical and Experimental Pathology (2017) IF: 1.706
    Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis.
    Sample: Cell culture supernatant
  21. Biology (2020)
    Induction of CD4 + CD25 + Regulatory T Cells from In Vitro Grown Human Mononuclear Cells by Sparteine Sulfate and Harpagoside

    DOI: 10.3390/biology9080211

    PMID: 32781652

    Sample: Cell culture supernatant
  22. Indian Journal of Public Health Research & Development (2019)
    Single Nucleotide Polymorphisms in IL-10-1082 A\G Gene and Association with HCMV Infection in Abortion

    DOI: 10.5958/0976-5506.2019.02580.4

    PMID: 99999999

    Sample: Serum
  23. International Journal of Research in Science and Technology (2020)
    THE REGULATION OF IMMUNE SYSTEM BY ANTI-INFLAMMATORY INTERLEUKINS IN HEART DISEASE PATIENTS INFECTED WITH TOXOPLASMOSIS

    DOI: 10.37648/ijrst.v10i02.003

    PMID: 99999999

    Sample: Serum
  24. Preprint (2020)
    Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma

    DOI: 10.21203/rs.3.rs-34263/v1

    PMID: 99999999

    Sample: Cell culture supernatant
  25. Biochemical and Cellular Archives (2017)
    ASSOCIATION OF IL-101082G/A GENE POLYMORPHISM WITH ITS Serum LEVELS IN ASTHMA PATIENTS

    PMID: 99999999

    Sample: Serum
  26. The Egyptian Rheumatologist (2018)
    CD4+ Foxp3+ T cells, interleukin-35 (IL-35) and IL-10 in systemic lupus erythematosus patients: Relation to disease activity

    DOI: 10.1016/j.ejr.2018.08.001

    PMID: 99999999

    Sample: Serum
  27. Iraqi Journal of Science (2018)
    Estimation of Levels of Interleukin-1 beta and Interleukin-10 in Sera of some Iraqi Patients with Chronic Rheumatoid Arthritis

    PMID: 99999999

    Sample: Serum
  28. Journal of THI-QAR SCIENCE (2017)
    The Relationship between Cytokines and Toxoplasma Infection in Heart Disease Patients.
    Sample: Serum
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