Human IL-6(Interleukin 6) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0102

      Synonyms:IL6, BSF2, HGF, HSF, IFNB2, CDF

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 7.81~500 pg/mL

      Sensitivity: 4.69 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 7.81—500 pg/mL
      Sensitivity 4.69 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      500 2.463
      2.487
      2.475 2.407
      250 1.612
      1.636
      1.624 1.556
      125 1.012
      0.978
      0.995 0.927
      62.5 0.513
      0.533
      0.523 0.455
      31.25 0.257
      0.245
      0.251 0.183
      15.63 0.174
      0.17
      0.172 0.104
      7.81 0.119
      0.123
      0.121 0.053
      0 0.067
      0.069
      0.068 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-6 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-6 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 25.30 67.60 210.10 24.20 66.50 197.40
      Standard deviation 1.70 2.80 7.80 1.60 3.40 6.70
      C V (%) 6.72 4.14 3.71 6.61 5.11 3.39

      Recovery

      The recovery of Human IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 97-110 102
      EDTA plasma (n=5) 92-108 99
      Cell culture media (n=5) 95-109 101

      Linearity

      Samples were spiked with high concentrations of Human IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 100-111 89-103 88-101
      Average (%) 105 97 93
      1:4 Range (%) 97-108 83-96 94-109
      Average (%) 102 90 100
      1:8 Range (%) 94-109 83-97 95-110
      Average (%) 101 89 102
      1:16 Range (%) 99-112 84-96 93-105
      Average (%) 106 88 99

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human.
        IF: 2.806
        Journal: Plos One(2013)
        DOI: 10.1371/journal.pone.0068963
        PMID: 23894384
        Products: E-EL-H0102 E-EL-H0109 
        Sample type: Plasma
        Reactivity: Human
      2. Plasma Levels Of C1q/TNF-Related Protein 1 And Interleukin 6 In Patients With Acute Coronary Syndrome Or Stable Angina Pectoris.
        IF: 1.847
        Journal: American Journal of The Medical Sciences(2015)
        DOI: 10.1097/MAJ.9999999900000378
        PMID: 25635749
        Products: E-EL-H0102 
        Sample type: Plasma
        Reactivity: Human
      3. Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages.
        IF: 3.365
        Journal: World Journal of Gastroenterology(2016)
        DOI: 10.3748/wjg.v22.i17.4354
        PMID: 27158204
        Products: E-EL-H0109 E-EL-H0102 E-EL-H0103 
        Sample type: Cell culture supernatant
        Reactivity: Human
      4. Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota.
        IF: 3.247
        Journal: Food & Function(2016)
        DOI: 10.1039/c6fo00831c
        PMID: 27713957
        Products: E-EL-H0102 E-EL-H0048 E-EL-H0149 
        Sample type: Plasma,Serum
        Reactivity: Human
      5. Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms.
        IF: 4.818
        Journal: Bioconjugate Chemistry(2016)
        DOI: 10.1021/acs.bioconjchem.6b00600
        PMID: 28004910
        Products: E-EL-H0109 E-EL-H0102 E-EL-H0103 
        Sample type: Cell culture supernatant
        Reactivity: Human
      6. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.
        IF: 3.834
        Journal: Plos Neglected Tropical Diseases(2016)
        DOI: 10.1371/journal.pntd.0004806
        PMID: 27348302
        Products: E-EL-H0099 E-EL-H0101 E-EL-H0102 E-EL-H0103 E-EL-H0108 
        Sample type: Serum,Cell culture supernatant
        Reactivity: Human
      7. In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10.
        IF: 3.833
        Journal: Apoptosis(2015)
        DOI: 10.1007/s10495-015-1162-9
        PMID: 26283170
        Products: E-EL-H0109 E-EL-H0102 E-EL-H0149 
        Sample type: Cell culture supernatant
        Reactivity: Human
      8. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation Through Both ROS Scavenging And Inhibition Of Cholesterol Influx In Raw264.7 Cells.
        IF: 2.861
        Journal: Molecules(2015)
        DOI: 10.3390/molecules201219764
        PMID: 26633327
        Products: E-EL-H0102 E-EL-H0109 
        Sample type: Cell lysate
        Reactivity: Human
      9. Applied Value Of Monitoring Serum Hepcidin In Differential Diagnosis Of Infection Versus Tumor Fevers.
        IF: 0.964
        Journal: Journal of Huazhong University of Science and Technology-Medical Sciences(2017)
        DOI: 10.1007/s11596-017-1724-x
        PMID: 28397053
        Products: E-EL-H0077 E-EL-H0102 
        Sample type: Plasma
        Reactivity: Human
      10. C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation.
        IF: 2.359
        Journal: Cardiovascular Pathology(2017)
        DOI: 10.1016/j.carpath.2017.03.009
        PMID: 28433861
        Products: E-EL-H0108 E-EL-H0109 E-EL-H0150 E-EL-H0102 
        Sample type: Cell culture supernatant
        Reactivity: Human
      11. Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy.
        IF: 13.942
        Journal: Acs Nano(2017)
        DOI: 10.1021/acsnano.6b06658
        PMID: 28107631
        Products: E-EL-H0109 E-EL-H0102 E-EL-H0088 
        Sample type: Cell culture supernatant
        Reactivity: Human
      12. The Classic Signalling And Trans-signalling Of Interleukin-6 Are Both Injurious In Podocyte Under High Glucose Exposure.
        IF: 4.499
        Journal: Journal of Cellular and Molecular Medicine(2017)
        DOI: 10.1111/jcmm.13314
        PMID: 28881473
        Products: E-EL-H0102 
        Sample type: Serum
        Reactivity: Human
      13. MicroRNA-218 Acts By Repressing TNFR1-Mediated Activation Of NF-κB, Which Is Involved In MUC5AC Hyper-Production And Inflammation In Smoking-Induced Bronchiolitis Of COPD.
        IF: 3.858
        Journal: Toxicology Letters(2017)
        DOI: 10.1016/j.toxlet.2017.08.079
        PMID: 28864214
        Products: E-EL-H2279 E-EL-H0102 E-EL-H0048 
        Sample type: Serum,Cell culture supernatant
        Reactivity: Human
      14. Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells.
        IF: 1.261
        Journal: Experimental and Therapeutic Medicine(2017)
        DOI: 10.3892/etm.2017.5053
        PMID: 29067127
        Products: E-EL-H0149 E-EL-H0102 E-EL-H0109 
        Sample type: Cell culture supernatant
        Reactivity: Human
      15. High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population.
        IF: 2.806
        Journal: Plos One(2017)
        DOI: 10.1371/journal.pone.0181440
        PMID: 28837559
        Products: E-EL-H0043 E-EL-H0109 E-EL-H0102 
        Sample type: Serum
        Reactivity: Human
      16. Depot-Specific Inflammation With Decreased Expression Of ATM2 In White Adipose Tissues Induced By High-Margarine/Lard Intake.
        IF: 2.806
        Journal: Plos One(2017)
        DOI: 10.1371/journal.pone.0188007
        PMID: 29141038
        Products: E-EL-H0102 E-EL-H0020 E-EL-H0052 E-EL-H1691 
        Sample type: Cell culture supernatant
        Reactivity: Human
      17. Particulate Matter 2.5 Regulates Lipid Synthesis And Inflammatory Cytokine Production In Human SZ95 Sebocytes.
        IF: 2.341
        Journal: International Journal of Molecular Medicine(2017)
        DOI: 10.3892/ijmm.2017.3109
        PMID: 28849137
        Products: E-EL-H0088 E-EL-H0102 E-EL-H0048 
        Sample type: Cell culture supernatant
        Reactivity: Human
      18. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation.
        IF: 2.759
        Journal: Biomedicine & Pharmacotherapy(2018)
        DOI: 10.1016/j.biopha.2017.11.151
        PMID: 29793333
        Products: E-EL-H5518 E-EL-H0755 E-EL-H0149 E-EL-H0109 E-EL-H0102 E-EL-RB0013 E-EL-RB0011 E-EL-RB0014 
        Sample type: Cell culture supernatant,Serum
        Reactivity: Human,Rabbit
      19. Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism.
        IF: 1.261
        Journal: Experimental and Therapeutic Medicine(2018)
        DOI: 10.3892/etm.2017.5593
        PMID: 29434746
        Products: E-EL-H0109 E-EL-H0149 E-EL-H0102 
        Sample type: Cell culture supernatant
        Reactivity: Human
      20. Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses.
        IF: 1.692
        Journal: Molecular Medicine Reports(2017)
        DOI: 10.3892/mmr.2017.7225
        PMID: 28849141
        Products: E-EL-H0109 E-EL-H0102 E-EL-H0149 
        Sample type: Cell culture supernatant
        Reactivity: Human
      21. Flexible Gold Electrode Array For Multiplexed Immunoelectrochemical Measurement Of Three Protein Biomarkers For Prostate Cancer.
        IF: 8.097
        Journal: ACS Applied Materials & Interfaces(2014)
        DOI: 10.1021/am505726b
        PMID: 25333408
        Products: E-EL-H0091 E-EL-H0102 E-EL-H5413 
        Reactivity: Human
      22. TSG Attenuates LPC-Induced Endothelial Cells Inflammatory Damage Through Notch Signaling Inhibition.
        IF: 3.141
        Journal: IUBMB Life(2016)
        DOI: 10.1002/iub.1458
        PMID: 26662286
        Products: E-EL-H0102 E-EL-H0043 
        Sample type: Cell culture supernatant
        Reactivity: Human
      23. TLR3-/4-Priming Differentially Promotes Ca2+Signaling And Cytokine Expression And Ca2+-Dependently Augments Cytokine Release In hMSCs.
        IF: 4.259
        Journal: Scientific Reports(2016)
        DOI: 10.1038/srep23103
        PMID: 26980664
        Products: E-EL-H0102 E-EL-H0048 E-EL-H0023 
        Sample type: Cell culture supernatant
        Reactivity: Human
      24. ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response.
        IF: 3.297
        Journal: Journal of molecular endocrinology(2018)
        DOI: 10.1530/JME-18-0078
        PMID: 30038054
        Products: E-EL-H0102 
        Sample type: Serum, Cell culture medium
        Reactivity: Human
      25. Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients.
        IF: 3.457
        Journal: Biomedicine & Pharmacotherapy(2018)
        DOI: 10.1016/j.biopha.2018.06.044
        PMID: 29966972
        Products: E-EL-H0102 E-EL-H0109 E-EL-H0043 E-EL-H5439 
        Sample type: Serum
        Reactivity: Human
      26. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura.
        IF: 0.533
        Journal: Pediatric Allergy(2018)
        DOI: 10.1089/ped.2018.0872
        Products: E-EL-H1554 E-EL-H0102 E-EL-H0048 E-EL-H0109 
        Sample type: Serum
        Reactivity: Human
      27. Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response.
        IF: /
        Journal: Medical Science Monitor: International Medical Journal of Experimental And Clinical Research(2018)
        DOI: 10.12659/MSM.908592
        PMID: 29940605
        Products: E-EL-H0109 E-EL-H0149 E-EL-H0102 
        Sample type: Serum
        Reactivity: Human
      28. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis.
        IF: 5.454
        Journal: Osteoarthritis and cartilage(2018)
        DOI: 10.1016/j.joca.2018.05.003
        PMID: 29753949
        Products: E-EL-H0109 E-EL-H0102 E-EL-H0048 E-EL-H5812 E-EL-H0253 
        Sample type: Serum
        Reactivity: Human
      29. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure
        IF: 5.714
        Journal: Environmental Pollution(2019)
        DOI: 10.1016/j.envpol.2019.06.059
        PMID: 31252119
        Products: E-EL-H0102 E-EL-H0048 
        Sample type: Cell culture supernatant
        Reactivity: Human
      30. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure
        IF: 5.714
        Products: E-EL-H0102 E-EL-H0048 
        Sample type: Cell supernatant
        Reactivity: Human
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