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Human IL-8(Interleukin 8) ELISA Kit

  • Cat.No.:E-EL-H6008

  • Reactivity: Human

To Purchase E-EL-H6008

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  • 96T
  • 24T
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-8. You can calculate the concentration of Human IL-8 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-8 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-8 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 20.34 60.67 245.62 24.62 56.15 233.52
Standard deviation 1.21 2.80 12.26 1.39 2.80 9.69
CV (%) 5.95 4.62 4.99 5.65 4.99 4.15

Recovery

The recovery of Human IL-8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 94
EDTA plasma (n=8) 95-102 98
Cell culture media (n=8) 98-106 102

Linearity

Samples were spiked with high concentrations of Human IL-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P10145
SynonymsIL8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, 3-10C, AMCF-I, K60
Research AreaCancer,Immunology,Cardiovascular

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell (2020) IF: 38.637
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009

    PMID: 99999999

    Sample: Cell culture medium
  2. Journal of Cellular Physiology (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  3. IF: 5.714
    Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure
    Sample: Cell supernatant
  4. Environmental Pollution (2019) IF: 5.714
    Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure

    DOI: 10.1016/j.envpol.2019.06.059

    PMID: 31252119

    Sample: Cell culture supernatant
  5. Osteoarthritis and cartilage (2018) IF: 5.454
    Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis.

    DOI: 10.1016/j.joca.2018.05.003

    PMID: 29753949

    Sample: Serum
  6. Nutrients (2020) IF: 4.546
    Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells

    DOI: 10.3390/nu12103064

    PMID: 33036498

    Sample: Cell culture supernatant
  7. Scientific Reports (2016) IF: 4.259
    TLR3-/4-Priming Differentially Promotes Ca2+Signaling And Cytokine Expression And Ca2+-Dependently Augments Cytokine Release In hMSCs.

    DOI: 10.1038/srep23103

    PMID: 26980664

    Sample: Cell culture supernatant
  8. International Journal of Nanomedicine (2017) IF: 4.3
    Zinc Oxide Nanoparticle-Induced Atherosclerotic Alterations In Vitro And In Vivo.

    DOI: 10.2147/IJN.S134897

    PMID: 28652743

    Sample: Cell culture supernatant,Serum
  9. Toxicology Letters (2017) IF: 3.858
    MicroRNA-218 Acts By Repressing TNFR1-Mediated Activation Of NF-κB, Which Is Involved In MUC5AC Hyper-Production And Inflammation In Smoking-Induced Bronchiolitis Of COPD.

    DOI: 10.1016/j.toxlet.2017.08.079

    PMID: 28864214

    Sample: Serum,Cell culture supernatant
  10. Febs Letters (2015) IF: 3.623
    The lncRNA MALAT1 Protects The Endothelium Against ox-LDL-Induced Dysfunction Via Upregulating The Expression Of The miR-22-3p Target Genes CXCR2 And AKT.

    DOI: 10.1016/j.febslet.2015.08.046

    PMID: 26364720

    Sample: Cell culture supernatant
  11. Food & Function (2016) IF: 3.247
    Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota.

    DOI: 10.1039/c6fo00831c

    PMID: 27713957

    Sample: Plasma,Serum
  12. Microbial Pathogenesis (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

    DOI: 10.1016/j.micpath.2020.104054

    PMID: 32061917

    Sample: Serum
  13. International Journal of Molecular Medicine (2017) IF: 2.341
    Particulate Matter 2.5 Regulates Lipid Synthesis And Inflammatory Cytokine Production In Human SZ95 Sebocytes.

    DOI: 10.3892/ijmm.2017.3109

    PMID: 28849137

    Sample: Cell culture supernatant
  14. Archives of Oral Biology (2020) IF: 1.931
    Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1

    DOI: 10.1016/j.archoralbio.2020.104858

    PMID: 32805637

    Sample: Cell culture supernatant
  15. Pediatric Allergy (2018) IF: 0.533
    High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura.

    DOI: 10.1089/ped.2018.0872

    Sample: Serum
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