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Human IRS1(Insulin Receptor Substrate 1) ELISA Kit

Cat:E-EL-H5554
Manual MSDS

Price: $ 609

Price: $ 487

Price: $ 150

Price: Inquire

Price: Inquire

Size:
96T 48T 24T 96T*10 96T*5
Quantity:
  • Reactivity: Human
  • Detection Range: 0.16-10 ng/mL
  • Sensitivity: 0.1 ng/mL
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For research use only. Order now, ship in 3 days

Product Details
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IRS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IRS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IRS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IRS1. You can calculate the concentration of Human IRS1 in the samples by comparing the OD of the samples to the standard curve.
Assay Type Sandwich-ELISA
size 96T / 48T / 24T / 96T*10 / 96T*5
Assay Time 3.5h
Detection Method Colormetric
Detection Range 0.16-10 ng/mL
Sensitivity 0.1 ng/mL
Sample Volume 100μL
Sample Type serum, plasma and other biological fluids
Specificity This kit recognizes Human IRS1 in samples. No significant cross-reactivity or interference between Human IRS1 and analogues was observed
Precision Both intra-CV and inter-CV are < 10%.
Recovery 80%-120%
Introduction This ELISA kit applies to the in vitro quantitative determination of Human IRS1 concentrations in serum, plasma and other biological fluids.
Applications ELISA
Storage 2-8℃/-20℃
  • Q1:What is the use of aluminum foil bag and plate sealer in the kit?

    In addition to the aluminum foil bag that has been vacuum-sealed, an empty aluminum foil bag will be included with the kit. Customers can seal and store the removed aluminum foil strip in a new aluminum foil bag and store the components according to the instructions. The plate sealer is a sticky film used to seal the plate holes in the incubation stage, which can control and reduce the evaporation of water in the incubation stage and better maintain the uniform heating of the whole plate, minimizing the influence of edge effect on the test results.

  • Q2:What are the specifications of ELISA Kit?

    There are four specifications of 96T/48T/24T/96T*5. Among them, 24T is the trial size.

  • Q3:Why do we recommend pre-testing? How to conduct a pre-experiment?

    The purpose of the pre-test is: ① to determine whether the kit is suitable for your sample detection, so as to avoid the waste of kits and samples; ② To help you get familiar with the operating process of the kit, especially the customers who use the ELISA kit for the first time/change the experimental brand; ③ Determine whether the sample is diluted or not and whether the dilution ratio is suitable for the determination of this kit and confirm the optimal sample dilution information; ④ Help to understand the experimental phenomena or problems that may occur during the experiment, so as to make timely adjustments; Specific methods: Before the formal test, 2-3 samples with large differences are selected to dilute the samples with different concentrations, and then pre-test is conducted according to the experimental procedures in the instructions. According to the results of the pre-test, the optimal dilution ratio of the samples is determined combined with the detection range of the kit.

  • Q4:Does ELISA require technical replication? How to set the positive or negative control?

    It is recommended that customers do technical repetition, on the one hand can confirm the operation method, on the other hand can verify and improve the accuracy of the experimental results. A positive control in an ELISA test can be considered the standard product. Negative control is a blank matrix or a matrix solution with known low concentration that has homology and homogeneity with the object to be picked up, which is generally difficult to obtain, and can be set as a negative control if available. The routine is to set a blank control, that is, a sample diluent.

  • Q5:What species can the ELISA Kit detect?

    At present, the species detected by our ELISA kit are mainly human, mouse, rat and a small number of targets involve special species such as monkey, rabbit, pig and chicken. Kits with the name of a particular species (such as human) are specific to that species. If no species is specified in the manual, the kit is generic within the conventional universal species. If the species tested is special, it is recommended to confirm the suitability of the sample with technical support first.

  • Q6:Why should a product with two item numbers of a target test different sample types?

    Different sample types of matrix are different, in order to reduce the influence of the matrix itself and improve the accuracy of routine sample measurement, usually set different systems of ELISA kits, and display with different product numbers. In addition, the concentration of some indicators varies greatly between different samples, so different products are used to meet customers' needs for ease of operation.

  • Q7:How to operate the "repeated freeze-thaw" process mentioned in tissue or cell sample processing?

    Tissue sample: After finishing tissue grinding, place it at -80℃ for 1h/ liquid nitrogen for 0.5h, and then gently shake it in a water bath at 30℃ to melt it quickly. Repeat this operation 1-2 times. Cell sample: Repeat the above freeze-thaw operation 2-3 times. If it is a membrane protein, it can be appropriately sonicated, but the temperature and frequency of ultrasound need to be controlled. It is recommended to add protease inhibitors to the sample in advance. PMSF(Cat.E-EL-SR002) is generally recommended.

  • Q8:Can the standard curve in the manual be used directly for calculation?

    The standard curve on the manual is an indicator curve, mainly used to show the shape of the standard curve after fitting and the quality control range of the highest/lowest OD value, you can not directly use. In addition, due to the influence of experimental operation, experimental environment, instrument parameter setting and other factors, the OD value of your actual standard curve may not be completely consistent with the instructions (overall high or low). In this case, it is recommended to control the first 4 blue wells in the color development stage with obvious color gradient and the maximum OD value of the standard curve above 1.2. If the correlation coefficient of the standard tune is above 0.99, it can be used as an effective standard curve.

  • Q9:How can I tell if my sample needs to be diluted or processed?

    The detection range of the kit is not the same as the concentration range of the substance to be measured in the sample. It is recommended to estimate the concentration of the substance to be measured in the sample through relevant literature before the experiment and determine the actual concentration of the sample through pre-experiment. If it is a routine sample type, you can consult ELISA technical support to obtain the recommended dilution of the sample and schedule pre-laboratory testing. If the concentration of the substance to be measured in the sample is too high or too low, dilute or concentrate the sample appropriately. If the concentration of analyte in the sample is much lower than the minimum detection line of the kit, it is recommended to choose a kit with higher sensitivity for detection.

  • Q10:The sample/reagent volume is not enough, can I reduce the sample volume uniformly?

    Neither can be reduced. The sample volume of each step should be strictly in accordance with the instructions, otherwise the experimental system will be changed, and the results will be inaccurate. If the customer really does not have enough sample volume, you can consult the technology to confirm whether the sample can be diluted, and judge the sample concentration through pre-experiment Degree situation. If the volume of the reagent is not enough, the number of holes to be tested can only be reduced according to the actual volume, and the detection can not be diluted.